Anthocyanin biosynthesis in pears is regulated by a R2R3-MYB transcription factor PyMYB10

被引:375
作者
Feng, Shouqian [1 ]
Wang, Yanling [1 ]
Yang, Song [1 ]
Xu, Yuting [1 ]
Chen, Xuesen [1 ]
机构
[1] Shandong Agr Univ, State Key Lab Crop Biol, Coll Hort, Tai An 271018, Shandong, Peoples R China
关键词
Anthocyanin; Asian pear; PyMYB10; Transcription factor; LOOP-HELIX PROTEIN; PETUNIA ENCODES; COLORFUL MODEL; DNA-BINDING; MYB DOMAIN; MAIZE C1; GENE; ARABIDOPSIS; IDENTIFICATION; FRUIT;
D O I
10.1007/s00425-010-1170-5
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Skin color is an important factor in pear breeding programs. The degree of red coloration is determined by the content and composition of anthocyanins. In plants, many MYB transcriptional factors are involved in regulating anthocyanin biosynthesis. In this study, a R2R3-MYB transcription factor gene, PyMYB10, was isolated from Asian pear (Pyrus pyrifolia) cv. 'Aoguan'. Sequence analysis suggested that the PyMYB10 gene was an ortholog of MdMYB10 gene, which regulates anthocyanin biosynthesis in red fleshed apple (Malus x domestica) cv. 'Red Field'. PyMYB10 was identified at the genomic level and had three exons, with its upstream sequence containing core sequences of cis-acting regulatory elements involved in light responsiveness. Fruit bagging showed that light could induce expression of PyMYB10 and anthocyanin biosynthesis. Quantitative real-time PCR revealed that PyMYB10 was predominantly expressed in pear skins, buds, and young leaves, and the level of transcription in buds was higher than in skin and young leaves. In ripening fruits, the transcription of PyMYB10 in the skin was positively correlated with genes in the anthocyanin pathway and with anthocyanin biosynthesis. In addition, the transcription of PyMYB10 and genes of anthocyanin biosynthesis were more abundant in red-skinned pear cultivars compared to blushed cultivars. Transgenic Arabidopsis plants overexpressing PyMYB10 exhibited ectopic pigmentation in immature seeds. The study suggested that PyMYB10 plays a role in regulating anthocyanin biosynthesis and the overexpression of PyMYB10 was sufficient to induce anthocyanin accumulation.
引用
收藏
页码:245 / 255
页数:11
相关论文
共 55 条
  • [1] The strawberry FaMYB1 transcription factor suppresses anthocyanin and flavonol accumulation in transgenic tobacco
    Aharoni, A
    De Vos, CHR
    Wein, M
    Sun, ZK
    Greco, R
    Kroon, A
    Mol, JNM
    O'Connell, AP
    [J]. PLANT JOURNAL, 2001, 28 (03) : 319 - 332
  • [2] The A locus that controls anthocyanin accumulation in pepper encodes a MYB transcription factor homologous to Anthocyanin2 of Petunia
    Borovsky, Y
    Oren-Shamir, M
    Ovadia, R
    De Jong, W
    Paran, I
    [J]. THEORETICAL AND APPLIED GENETICS, 2004, 109 (01) : 23 - 29
  • [3] Transcription factors as tools for metabolic engineering in plants
    Broun, P
    [J]. CURRENT OPINION IN PLANT BIOLOGY, 2004, 7 (02) : 202 - 209
  • [4] Chang S. J., 1993, Plant Molecular Biology Reporter, V11, P113, DOI 10.1007/BF02670468
  • [5] Floral dip:: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana
    Clough, SJ
    Bent, AF
    [J]. PLANT JOURNAL, 1998, 16 (06) : 735 - 743
  • [6] DAYTON DF, 1966, P AM SOC HORTIC SCI, V89, P110
  • [7] The an11 locus controlling flower pigmentation in petunia encodes a novel WD-repeat protein conserved in yeast, plants, and animals
    deVetten, N
    Quattrocchio, F
    Mol, J
    Koes, R
    [J]. GENES & DEVELOPMENT, 1997, 11 (11) : 1422 - 1434
  • [8] CHARACTERIZING AND QUANTIFYING ANTHOCYANINS IN RED PEARS AND THE EFFECT OF LIGHT QUALITY ON FRUIT COLOR
    DUSSI, MC
    SUGAR, D
    WROLSTAD, RE
    [J]. JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE, 1995, 120 (05) : 785 - 789
  • [9] Red colouration in apple fruit is due to the activity of the MYB transcription factor, MdMYB10
    Espley, Richard V.
    Hellens, Roger P.
    Putterill, Jo
    Stevenson, David E.
    Kutty-Amma, Sumathi
    Allan, Andrew C.
    [J]. PLANT JOURNAL, 2007, 49 (03) : 414 - 427
  • [10] Multiple Repeats of a Promoter Segment Causes Transcription Factor Autoregulation in Red Apples
    Espley, Richard V.
    Brendolise, Cyril
    Chagne, David
    Kutty-Amma, Sumathi
    Green, Sol
    Volz, Richard
    Putterill, Jo
    Schouten, Henk J.
    Gardiner, Susan E.
    Hellens, Roger P.
    Allan, Andrew C.
    [J]. PLANT CELL, 2009, 21 (01) : 168 - 183