Detection of pathogenic Streptococcus suis bacteria using magnetic glycoparticles

被引：33

作者：

Pera, Nuria Parera ^{[1
]}

Kouki, Annika ^{[2
]}

Haataja, Sauli ^{[2
]}

Branderhorst, Hilbert M. ^{[1
]}

Liskamp, Rob M. J. ^{[1
]}

Visser, Gerben M. ^{[3
]}

Finne, Jukka ^{[4
]}

Pieters, Roland J. ^{[1
]}

机构：

[1] Univ Utrecht, Dept Med Chem & Chem Biol, Utrecht Inst Pharmaceut Sci, NL-3508 TB Utrecht, Netherlands

[2] Univ Turku, Dept Med Biochem & Genet, FI-20520 Turku, Finland

[3] Wageningen Univ, Organ Chem Lab, NL-6703 HB Wageningen, Netherlands

[4] Univ Helsinki, Dept Biosci, FI-00014 Helsinki, Finland

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2010年 / 8卷 / 10期

关键词：

ESCHERICHIA-COLI; ADHESION; RECOGNITION; NANOPARTICLES; INHIBITION;

D O I：

10.1039/c000819b

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Detection of the zoonotic bacterial pathogen Streptococcus suis was achieved using magnetic glycoparticles. The bacteria contain an adhesion protein for the carbohydrate sequence Gal alpha 1,4Gal. After incubation with various amounts of the pathogen, magnetic concentration and ATP detection, bacterial levels down to 10(5) cfu could be detected. Submicrometer particles were needed, since with the larger microparticles the method did not succeed.

引用

页码：2425 / 2429

页数：5

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