Detection and expression of a gene encoding a new bacteriorhodopsin from an extreme halophile strain HT (JCM 9743) which does not possess bacteriorhodopsin activity

被引:17
作者
Kamekura, M
Seno, Y
Tomioka, H
机构
[1] Noda Inst Sci Res, Noda, Chiba 278, Japan
[2] Saitama Univ, Fac Educ, Dept Chem, Urawa, Saitama 338, Japan
关键词
bacteriorhodopsin; extreme halophile; Halobacterium salinarum; vector pXLNov-r; photocycle;
D O I
10.1007/s007920050040
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Membrane vesicles prepared from an extreme halophile strain, HT (JCM 9743), showed no bacteriorhodopsin activity. However, a DNA fragment, amplified by polymerase chain reaction (PCR), appeared to encode the C to G helices of a bacteriorhodopsin(bR)-like protein. With the PCR product as a probe, the gene coding for a novel bacteriorhodopsin was cloned from the genomic DNA of the strain HT. The open reading frame of the gene was ligated with the promoter region of the bop gene of Halobacterium salinarum bR, and expressed in a bR-deficient host strain, L33, using the plasmid vector pXLNov-R. The purplish membrane fraction purified from cells of a transformant exhibited a cyclic photoreaction characteristic of bacteriorhodopsin.
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页码:33 / 39
页数:7
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