Ultrastructural changes associated with cryopreservation of banana (Musa spp.) highly proliferating meristems

被引：76

作者：

Helliot, B

Swennen, R

Poumay, Y

Frison, E

Lepoivre, P ^{[1
]}

Panis, B

机构：

[1] Gembloux Agr Univ, Plant Pathol Unit, B-5030 Gembloux, Belgium

[2] Katholieke Univ Leuven, Lab Trop Crop Improvement, B-3001 Louvain, Belgium

[3] Fac Univ Notre Dame Paix, Dept Histol & Embryol, B-5000 Namur, Belgium

[4] IPGRI, INIBAP, F-34397 Montpellier, France

来源：

PLANT CELL REPORTS | 2003年 / 21卷 / 07期

关键词：

banana streak virus; cucumber mosaic virus; cryopreservation; electron microscopy; Musa;

D O I：

10.1007/s00299-002-0537-x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Cryopreservation has been shown to improve the frequency of virus elimination - specifically cucumber mosaic virus and banana streak virus - from banana (Musa spp.) plants. To understand the mode of action of cryopreservation for the eradication of viral particles, we examined the ultrastructure of meristem tips at each step of the cryopreservation process. Excised meristematic clumps produced from infected banana plants belonging to cv. Williams (AAA, Cavendish subgroup) were cryopreserved through vitrification using the PVS-2 solution. We demonstrated that the cryopreservation method used only allowed survival of small areas of cells in the meristematic dome and at the base of the primordia. Cellular and subcellular changes occurring during the cryopreservation process are discussed.

引用

页码：690 / 698

页数：9

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