Isolation and mutation site determination of the temperature-sensitive murB mutants of Staphylococcus aureus

被引：25

作者：

Matsuo, M

Kurokawa, K

Nishida, S

Lu, Y

Takimura, H

Kaito, C

Fukuhara, N

Maki, H

Miura, K

Murakami, K

Sekimizu, K

机构：

[1] Univ Tokyo, Grad Sch Pharmaceut Sci, Bunkyo Ku, Tokyo 1130033, Japan

[2] Shionogi & Co Ltd, Discovery Res Labs, Toyonaka, Osaka 5610825, Japan

来源：

FEMS MICROBIOLOGY LETTERS | 2003年 / 222卷 / 01期

关键词：

Staphylococcus aureus; murB; UDP-N-acetylenolpyruvylglucosamine reductase; temperature-sensitive mutant; murein; peptidoglycan;

D O I：

10.1016/S0378-1097(03)00260-X

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The murB gene encodes UDP-N-acetylenolpyruvylglucosamine reductase and functions in bacterial peptidoglycan biosynthesis. A plasmid carrying the murB gene restored the temperature-sensitive growth of six Staphylococcus aureus mutants, in which peptidoglycan biosynthesis stopped at a restrictive temperature. Specific activity of UDP-N-acetylenolpyruvylglucosamine reductase in extracts from the mutants was lower than that from wild-type cells. Nucleotide sequence determination revealed that each mutant had a single amino acid substitution in the mur-B gene and five of six mutations were located within domain 3, where the proposed substrate binding site is located. These results suggest that the murB gene is essential for growth of S. aureus and that domain 3 is important for the MurB activity. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：107 / 113

页数：7

共 22 条

[1] AN ENZYME-SUBSTRATE COMPLEX INVOLVED IN BACTERIAL-CELL WALL BIOSYNTHESIS [J].