Identification of a truncated Tn1721-like transposon located on a small plasmid of Escherichia coli isolated from Varanus indicus

被引：7

作者：

Waturangi, DE ^{[1
]}

Schwarz, S ^{[1
]}

Suwanto, A ^{[1
]}

Kehrenberg, C ^{[1
]}

Erdelen, W ^{[1
]}

机构：

[1] Bundesforsch Anstalt Landwirtschaft FAL, Inst Tierzucht, D-31535 Neustadt, Germany

来源：

JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH | 2003年 / 50卷 / 02期

关键词：

D O I：

10.1046/j.1439-0450.2003.00625.x

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The 9.1 kb plasmid pDEWT1 was isolated from an Escherichia coli strain obtained from the faeces of a free-living lizard (Varanus indicus ) in Indonesia. This plasmid mediated tetracycline resistance via a tet gene of hybridization class A. Molecular analysis of a 7755 bp segment of plasmid pDEWT1 including the tetR-tet (A) region and its flanking areas suggested that pDEWT1 harboured a truncated copy of the tet (A)-carrying transposon Tn1721 in which the part responsible for chemotaxis and transposition functions was lost. Analysis of the sequences at the integration site revealed the presence of the 5-bp direct repeat TACTT. The sequences upstream and downstream of the integration site showed striking homology to sequences of a non-coding region detectable on a small cryptic plasmid from Yersinia enterocolitica .

引用

页码：86 / 89

页数：4

共 25 条

[1] COMPLETE NUCLEOTIDE-SEQUENCE OF TN1721 - GENE ORGANIZATION AND A NOVEL GENE-PRODUCT WITH FEATURES OF A CHEMOTAXIS PROTEIN [J].

ALLMEIER, H ;

CRESNAR, B ;

GRECK, M ;

SCHMITT, R .

GENE, 1992, 111 (01) :11-20

[2]

ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999

[3] PROPERTIES OF 2 CONJUGATIVE PLASMIDS MEDIATING TETRACYCLINE RESISTANCE, RAFFINOSE CATABOLISM AND HYDROGEN-SULFIDE PRODUCTION IN ESCHERICHIA-COLI [J].

BURKARDT, HJ ;

MATTES, R ;

SCHMID, K ;

SCHMITT, R .

MOLECULAR AND GENERAL GENETICS, 1978, 166 (01) :75-84

[4] Complete nucleotide sequence of Tn10 [J].

Chalmers, R ;

Sewitz, S ;

Lipkow, K ;

Crellin, P .

JOURNAL OF BACTERIOLOGY, 2000, 182 (10) :2970-2972

[5] Tetracycline antibiotics: Mode of action, applications, molecular biology, and epidemiology of bacterial resistance [J].

Chopra, I ;

Roberts, M .

MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 2001, 65 (02) :232-+

[6] Tetracycline resistance in Salmonella enterica subsp. enterica serovar Dublin [J].

Frech, G ;

Schwarz, S .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1998, 42 (05) :1288-1289

[7] Molecular analysis of tetracycline resistance in Salmonella enterica subsp enterica serovars Typhimurium, Enteritidis, Dublin, Choleraesuis, Hadar and Saintpaul:: construction and application of specific gene probes [J].

Frech, G ;

Schwarz, S .

JOURNAL OF APPLIED MICROBIOLOGY, 2000, 89 (04) :633-641

[8]

Frech G, 1999, FEMS MICROBIOL LETT, V176, P97, DOI [10.1016/S0378-1097(99)00226-8, 10.1111/j.1574-6968.1999.tb13648.x]

[9] PCR typing of tetracycline resistance determinants (Tet A-E) in Salmonella enterica serotype Hadar and in the microbial community of activated sludges from hospital and urban wastewater treatment facilities in Belgium [J].

Guillaume, G ;

Verbrugge, D ;

Chasseur-Libotte, ML ;

Moens, W ;

Collard, JM .

FEMS MICROBIOLOGY ECOLOGY, 2000, 32 (01) :77-85

[10] Tn5706, a transposon-like element from Pasteurella multocida mediating tetracycline resistance [J].

Kehrenberg, C ;

Werckenthin, C ;

Schwarz, S .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1998, 42 (08) :2116-2118

← 1 2 3 →