Identification of a truncated Tn1721-like transposon located on a small plasmid of Escherichia coli isolated from Varanus indicus

被引:7
作者
Waturangi, DE [1 ]
Schwarz, S [1 ]
Suwanto, A [1 ]
Kehrenberg, C [1 ]
Erdelen, W [1 ]
机构
[1] Bundesforsch Anstalt Landwirtschaft FAL, Inst Tierzucht, D-31535 Neustadt, Germany
来源
JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH | 2003年 / 50卷 / 02期
关键词
D O I
10.1046/j.1439-0450.2003.00625.x
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
The 9.1 kb plasmid pDEWT1 was isolated from an Escherichia coli strain obtained from the faeces of a free-living lizard (Varanus indicus ) in Indonesia. This plasmid mediated tetracycline resistance via a tet gene of hybridization class A. Molecular analysis of a 7755 bp segment of plasmid pDEWT1 including the tetR-tet (A) region and its flanking areas suggested that pDEWT1 harboured a truncated copy of the tet (A)-carrying transposon Tn1721 in which the part responsible for chemotaxis and transposition functions was lost. Analysis of the sequences at the integration site revealed the presence of the 5-bp direct repeat TACTT. The sequences upstream and downstream of the integration site showed striking homology to sequences of a non-coding region detectable on a small cryptic plasmid from Yersinia enterocolitica .
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页码:86 / 89
页数:4
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