Matrix metaltoproteinases and their inhibitors in the cervical mucus plug at term of pregnancy

被引：19

作者：

Becher, N ^{[1
]}

Hein, M

Danielsen, CC

Uldbjerg, N

机构：

[1] Univ Aarhus, Skejby Hosp, Lab Obstet & Gynecol, DK-8200 Aarhus N, Denmark

[2] Univ Aarhus, Inst Anat, Dept Connect Tissue Biol, Aarhus, Denmark

来源：

AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY | 2004年 / 191卷 / 04期

关键词：

cervical mucus plug; pregnancy; matrix metalloproteinase-2; matrix metalloproteinase-8; matrix metalloproteinase-9; tissue inhibitor of matrix metalloproteinase-1;

D O I：

10.1016/j.ajog.2004.03.023

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Objective: This study was undertaken to evaluate the presence of matrix (MMPs) and their inhibitors in the cervical mucus plug obtained during active labor at term. Study design: Cervical mucus plugs from 17 healthy women in normal active labor were homogenized, extracted, and analyzed by 4 different assays. Results: Gelatin zymography revealed large amounts of MMP-2 and MMP-9, and triple-helical collagen degradation demonstrated collagenase activity (MMP-8). Reverse zymography showed that tissue inhibitor of matrix metalloproteinase I (TIMP-1) and TIMP-2 are detectable in the cervical mucus plug. Quantification by enzyme-linked immunosorbent assay confirmed the presence of MMP-2, MMP-8, MMP-9, and TIMP-1. Conclusion: Our results suggest that proteolytic or nonproteolytic effects of MMP and TIMP may play a part in the function of the cervical mucus plug of pregnancy. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：1232 / 1239

页数：8

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