Over expression of the yeast BFR2 gene partially suppresses the growth defects induced by Brefeldin A and by four ER-to-Golgi mutations

被引:4
作者
Chabane, S [1 ]
Gachet, E [1 ]
Képès, F [1 ]
机构
[1] CEA Saclay, Serv Biochim & Genet Mol, DBCM, DSV, F-91191 Gif Sur Yvette, France
关键词
Saccharomyces cerevisiae; protein transport; Golgi apparatus; Brefeldin A;
D O I
10.1007/s002940050304
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The fungal metabolite Brefeldin A (BFA) disrupts the Golgi apparatus and its incoming protein flux. We developed a genetic approach to identify yeast proteins involved in the protein transport step that BFA blocks. The BFR2 gene (YDR299W) was thus isolated as a high-copy suppressor of the growth defects induced by BFA in a sensitive strain of Saccharomyces cerevisiae. Although BFR2 over-expression did not cause a secretory block or slow-down, it partially suppressed the growth defect of four mutants blocked at the step of budding or docking of small vesicles en route to the Golgi (sec13-1, sec16-2, sec23-1, ypt1-1). The essential BFR2 gene was predicted to encode an extremely hydrophilic product containing two short regions with potential coiled-coils, one of which corresponds to a cluster of acidic residues.
引用
收藏
页码:21 / 28
页数:8
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