Behaviour of human mesenchymal stem cells on chemically synthesized HA-PCL scaffolds for hard tissue regeneration

被引：36

作者：

D'Anto, Vincenzo ^{[1
,2
,3
]}

Raucci, Maria Grazia ^{[1
]}

Guarino, Vincenzo ^{[1
]}

Martina, Stefano ^{[2
]}

Valletta, Rosa ^{[2
]}

Ambrosio, Luigi ^{[1
]}

机构：

[1] CNR, Natl Res Council Italy, Inst Composite & Biomed Mat IMCB, P Le Tecchio 80, I-80125 Naples, Italy

[2] Univ Naples Federico II, Dept Neurosci Reprod Sci & Oral Sci, Naples, Italy

[3] Bambino Gesu Pediat Hosp, Dept Pediat Surg & Transplantat, Rome, Italy

来源：

JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE | 2016年 / 10卷 / 02期

关键词：

composite material; hydroxyapatite; polymer; scaffold; human mesenchymal stem cells; dental pulp stem cells; bone defects; MARROW STROMAL CELLS; SOL-GEL SYNTHESIS; TRICALCIUM PHOSPHATE; IN-VITRO; OSTEOGENIC DIFFERENTIATION; BONE REPAIR; HYDROXYAPATITE; HYBRID; BIOACTIVITY; PARTICLES;

D O I：

10.1002/term.1768

中图分类号：

Q813 [细胞工程];

学科分类号：

100113 [医学细胞生物学];

摘要：

Our goal was to characterize the response of human mesenchymal stem cells (hMSCs) to a novel composite scaffold for bone tissue engineering. The hydroxyapatite-polycaprolactone (HA-PCL) composite scaffolds were prepared by a sol-gel method at room temperature and the scaffold morphology was investigated by scanning electron microscopy (SEM)/energy-dispersive spectroscopy (EDS) to validate the synthesis process. The response of two different lines of hMSCs, bone-marrow-derived human mesenchymal stem cells (BMSCs) and dental pulp stem cells (DPSCs) in terms of cell proliferation and differentiation into the osteoblastic phenotype, was evaluated using Alamar blue assay, SEM, histology and alkaline phosphatase activity. Our results indicate that tissue engineering by means of composite HA-PCL scaffolds may represent a new therapeutic strategy to repair craniofacial bone defects. Copyright (c) 2013 John Wiley & Sons, Ltd.

引用

页码：E147 / E154

页数：8

共 38 条

[1]

SPARC, a matricellular protein that functions in cellular differentiation and tissue response to injury [J].

Bradshaw, AD ;

Sage, EH .

JOURNAL OF CLINICAL INVESTIGATION, 2001, 107 (09) :1049-1054

[2]

A tissue engineering approach to bone repair in large animal models and in clinical practice [J].

Cancedda, Ranieri ;

Giannoni, Paolo ;

Mastrogiacomo, Maddalena .

BIOMATERIALS, 2007, 28 (29) :4240-4250

[3]

Catauro M, 2007, J APPL BIOMATER BIOM, V5, P41

[4]

Sol-gel synthesis, structure and bioactivity of polycaprolactone/CaO•SiO2 hybrid material [J].

Catauro, M ;

Raucci, MG ;

De Gaetano, F ;

Buri, A ;

Marotta, A ;

Ambrosio, L .

JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 2004, 15 (09) :991-995

[5]

Osteonectin-null mutation compromises osteoblast formation, maturation, and survival [J].

Delany, AM ;

Kalajzic, I ;

Bradshaw, AD ;

Sage, EH ;

Canalis, E .

ENDOCRINOLOGY, 2003, 144 (06) :2588-2596

[6]

Design of injectable organic-inorganic hybrid for bone tissue repair [J].

Dessi, Mariagemiliana ;

Raucci, Maria Grazia ;

Zeppetelli, Stefania ;

Ambrosio, Luigi .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2012, 100A (08) :2063-2070

[7]

Outcome of acetabular revision using an uncemented hydroxyapatite-coated component - Two- to five-year results and review [J].

Dorairajan, A ;

Reddy, RM ;

Krikler, S .

JOURNAL OF ARTHROPLASTY, 2005, 20 (02) :209-218

[8]

Fabrication of a three-dimensional nanostructured biomaterial for tissue engineering of bone [J].

Garreta, E. ;

Gasset, D. ;

Semino, C. ;

Borros, S. .

BIOMOLECULAR ENGINEERING, 2007, 24 (01) :75-80

[9]

Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo [J].

Gronthos, S ;

Mankani, M ;

Brahim, J ;

Robey, PG ;

Shi, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (25) :13625-13630

[10]

Bioactive scaffolds for bone and ligament tissue [J].

Guarino, Vincenzo ;

Causa, Filippo ;

Ambrosio, Luigi .

EXPERT REVIEW OF MEDICAL DEVICES, 2007, 4 (03) :405-418

← 1 2 3 4 →