Comparison of 3 Selective Media for Enumeration of Bacillus cereus in Several Food Matrixes

被引：11

作者：

Chon, Jung-Whan ^{[1
]}

Song, Kwang-Young ^{[1
]}

Kim, Hyunsook ^{[1
]}

Seo, Kun-Ho ^{[1
]}

机构：

[1] Konkuk Univ, KU Ctr Food Safety, Seoul 143701, South Korea

来源：

JOURNAL OF FOOD SCIENCE | 2014年 / 79卷 / 12期

关键词：

Bacillus cereus; background microflora; comparison; food matrix; selective agars; REAL-TIME PCR; POLYMYXIN-B; CULTURE METHOD; PLATING MEDIA; IDENTIFICATION; TRIMETHOPRIM; AGAR; STANDARD; ASSAY; SPP;

D O I：

10.1111/1750-3841.12594

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

In this study, we compared the inclusivity, exclusivity, recoverability, and selectivity of the 3 selective agars (mannitol yolk polymyxin B agar [MYPA], polymyxin pyruvate egg yolk mannitol bromothymol blue agar [PEMBA], and Brillance Bacillus cereus agar [BBC agar]) for Bacillus cereus (B. cereus) from pure culture and several food samples. BBC agar showed greater exclusivity and selectivity in pure culture and in foods with high background flora, respectively; however, all the tested media showed similar recoverability (P > 0.05) of B. cereus in pure culture and in most foods. Our results suggest that BBC agar could be useful to enumerate B. cereus from, in particular, food matrixes with high background competing micro flora.

引用

页码：M2480 / M2484

页数：5

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