Tryptophan determination in proteins and feedstuffs by ion exchange chromatography

被引：69

作者：

Ravindran, G ^{[1
]}

Bryden, WL ^{[1
]}

机构：

[1] Univ Sydney, Dept Anim Sci, Camden, NSW 2570, Australia

来源：

FOOD CHEMISTRY | 2005年 / 89卷 / 02期

关键词：

tryptophan; proteins; ion-exchange chromatography; alkaline hydrolysis;

D O I：

10.1016/j.foodchem.2004.05.035

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A chromatographic method was developed for the determination of tryptophan content in food and feed proteins. The method involves separation and quantitation of tryptophan (released from protein by alkaline hydrolysis with NaOH) by isocratic ion-exchange chromatography with O-phthalaldehyde derivatization followed by fluorescence detection. In this procedure, chromatographic separation of the tryptophan and alpha-methyl tryptophan, the internal standard, was complete in 15 min, without any interference from other compounds. The precision of the method was 1-4%, relative standard deviation. Accuracy was validated by agreement with the value for chicken egg white lysozyme, a sequenced protein, and by quantitative recoveries after spiking with lysozyme. The method allows determination in a range of feed proteins, containing varied concentrations of tryptophan, and is applicable to systems used for routine amino acid analysis by ion-exchange chromatography. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：309 / 314

页数：6

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