Structure characterization and antioxidant activity of a novel polysaccharide isolated from Ginkgo biloba

被引：88

作者：

Yuan, Fei ^{[1
]}

Yu, Rongmin ^{[1
]}

Yin, Yin ^{[1
]}

Shen, Jianru ^{[1
]}

Dong, Quanfeng ^{[1
]}

Zhong, Ling ^{[2
]}

Song, Liyan ^{[2
]}

机构：

[1] Jinan Univ, Dept Nat Prod Chem, Guangzhou 510632, Guangdong, Peoples R China

[2] Jinan Univ, Dept Pharmacol, Guangzhou 510632, Guangdong, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2010年 / 46卷 / 04期

关键词：

Polysaccharide of Ginkgo biloba; Structure characterization; Antioxidant activity; FRUITING BODY; ELUCIDATION; EXTRACTION;

D O I：

10.1016/j.ijbiomac.2010.02.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

A novel polysaccharide (GBP5052) with antioxidant activity was isolated from Ginkgo biloba. The structure of GBP5052 was elucidated on the basis of physico-chemical and instrumental analyses, and its average molecular weight (Mw = 2 30 x 10(5)) was determined by gel permeation chromatography. The backbone of GBP5052 was composed of (1 -> 4)-linked alpha-D-mannopyranosyl residues which branched at O-3. The three branches consisted of beta-L-rhamnopyranosyl residues, (1 -> 4)-linked alpha-D-galactopyranosyl terminated with beta-L-rhamnopyranosyl residues, and (1 -> 3,4)-linked alpha-D-mannopyranosyl terminated with beta-L-rhamnopyranosyl residues, respectively. In the in vitro antioxidant assay, GBP5052 was found to possess DPPH radical-scavenging activity and hydroxyl radical-scavenging activity with an IC50 value of 0 412 mg/mL and 0.482 mg/mL, respectively. (C) 2010 Elsevier B V All rights reserved

引用

页码：436 / 439

页数：4

共 24 条

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