Cell biology of laryngeal epithelial defenses in health and disease: Further studies

被引:140
作者
Johnston, N
Bulmer, D
Gill, GA
Panetti, M
Ross, PE
Pearson, JP
Pignatelli, M
Axford, SE
Dettmar, PW
Koufman, JA
机构
[1] Wake Forest Univ, Sch Med, Dept Otolaryngol, Ctr Voice Disorders, Winston Salem, NC 27157 USA
[2] Univ Newcastle Upon Tyne, Sch Med, Dept Physiol Sci, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England
[3] Univ Bristol, Sch Med Sci, Dept Pathol & Microbiol, Bristol BS8 1TD, Avon, England
[4] Univ Dundee, Ninewells Hosp & Med Sch, Dept Mol & Cellular Pathol, Dundee DD1 9SY, Scotland
关键词
carbonic anhydrase; cell biology; cellular defenses; E-cadherin; extraesophageal reflux; gastroesophageal reflux; laryngopharyngeal reflux; mucin; mucus; reflux; vocal fold;
D O I
10.1177/000348940311200601
中图分类号
R76 [耳鼻咽喉科学];
学科分类号
100213 ;
摘要
This is the second annual report of an international collaborative research group that is examining the cellular impact of laryngopharyngeal reflux (LPR) on laryngeal epithelium. The results of clinical and experimental studies are presented. Carbonic anhydrase (CA), E-cadherin, and MUC gene expression were analyzed in patients with LPR, in controls, and in an in vitro model. In patients with LPR, we found decreased levels of CAIII in vocal fold epithelium and increased levels in posterior commissure epithelium. The experimental studies confirm that laryngeal CAIII is depleted in response to reflux. Also, cell damage does occur well above pH 4.0. In addition, E-cadherin (transmembrane cell surface molecules, which have a key function in epithelial cell adhesion) was not present in 37% of the LPR laryngeal specimens. In conclusion, the laryngeal epithelium lacks defenses comparable to those in esophageal epithelium, and these differences may contribute to the increased susceptibility of laryngeal epithelium to reflux-related injury.
引用
收藏
页码:481 / 491
页数:11
相关论文
共 60 条
[31]   ACID POSTERIOR LARYNGITIS - ETIOLOGY, HISTOLOGY, DIAGNOSIS AND TREATMENT [J].
KAMBIC, V ;
RADSEL, Z .
JOURNAL OF LARYNGOLOGY AND OTOLOGY, 1984, 98 (12) :1237-1240
[32]   INTERMEDIATE FILAMENTS - NEW PROTEINS, SOME ANSWERS, MORE QUESTIONS [J].
KLYMKOWSKY, MW .
CURRENT OPINION IN CELL BIOLOGY, 1995, 7 (01) :46-54
[33]  
Koufman JA, 1997, OTOLARYNG CLIN N AM, V30, P1
[34]   Prevalence of reflux in 113 consecutive patients with laryngeal and voice disorders [J].
Koufman, JA ;
Amin, MR ;
Panetti, M .
OTOLARYNGOLOGY-HEAD AND NECK SURGERY, 2000, 123 (04) :385-388
[35]   Laryngopharyngeal reflux: Position statement of the committee on speech, voice, and swallowing disorders of the American Academy of Otolaryngology-Head and Neck Surgery [J].
Koufman, JA ;
Aviv, JE ;
Casiano, RR ;
Shaw, GY .
OTOLARYNGOLOGY-HEAD AND NECK SURGERY, 2002, 127 (01) :32-35
[36]  
KOUFMAN JA, 1991, LARYNOSCOPE S53, V101
[37]   Symptomatic gastroesophageal reflux as a risk factor for esophageal adenocarcinoma [J].
Lagergren, J ;
Bergström, R ;
Lindgren, A ;
Nyrén, O .
NEW ENGLAND JOURNAL OF MEDICINE, 1999, 340 (11) :825-831
[38]  
LARIVEE P, 1994, AIRWAY SECRETION PHY, P469
[39]   Acid-induced laryngospasm in a canine model [J].
Loughlin, CJ ;
Koufman, JA ;
Averill, DB ;
Cummins, MM ;
Kim, YJ ;
Little, JP ;
Miller, IJ ;
Meredith, JW .
LARYNGOSCOPE, 1996, 106 (12) :1506-1509
[40]   Paroxysmal laryngospasm secondary to gastroesophageal reflux [J].
Loughlin, CJ ;
Koufman, JA .
LARYNGOSCOPE, 1996, 106 (12) :1502-1505