共 37 条
Physcomitrella patens mutants affected on heat dissipation clarify the evolution of photoprotection mechanisms upon land colonization
被引:151
作者:
Alboresi, Alessandro
[1
]
Gerotto, Caterina
[2
]
Giacometti, Giorgio M.
[2
]
Bassi, Roberto
[1
]
Morosinotto, Tomas
[2
]
机构:
[1] Univ Verona, Dipartimento Biotecnol, I-37134 Verona, Italy
[2] Univ Padua, Dipartimento Biol, I-35121 Padua, Italy
来源:
关键词:
LHCSR;
nonphotochemical quenching;
photosystem;
plants evolution;
PSBS;
PHOTOSYSTEM-II ANTENNA;
CHLOROPHYLL FLUORESCENCE;
LIGHT;
PSBS;
IDENTIFICATION;
PROTEIN;
MOSS;
DNA;
SYSTEMS;
ORIGIN;
D O I:
10.1073/pnas.1002873107
中图分类号:
O [数理科学和化学];
P [天文学、地球科学];
Q [生物科学];
N [自然科学总论];
学科分类号:
07 ;
0710 ;
09 ;
摘要:
Light is the source of energy for photosynthetic organisms; when in excess, however, it also drives the formation of reactive oxygen species and, consequently, photoinhibition. Plants and algae have evolved mechanisms to regulate light harvesting efficiency in response to variable light intensity so as to avoid oxidative damage. Nonphotochemical quenching (NPQ) consists of the rapid dissipation of excess excitation energy as heat. Although widespread among oxygenic photosynthetic organisms, NPQ shows important differences in its machinery. In land plants, such as Arabidopsis thaliana, NPQ depends on the presence of PSBS, whereas in the green alga Chlamydomonas reinhardtii it requires a different protein called LHCSR. In this work, we show that both proteins are present in the moss Physcomitrella patens. By generating KO mutants lacking PSBS and/or LHCSR, we also demonstrate that both gene products are active in NPQ. Plants lacking both proteins are more susceptible to high light stress than WT, implying that they are active in photoprotection. These results suggest that NPQ is a fundamental mechanism for survival in excess light and that upon land colonization, photosynthetic organisms evolved a unique mechanism for excess energy dissipation before losing the ancestral one found in algae.
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页码:11128 / 11133
页数:6
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