Measurement of Poly(ethylene glycol) by Cell-Based Anti-poly(ethylene glycol) ELISA

Poly(ethylene glycol) (PEG) is increasingly used in clinical and experimental medicine. However, quantification of PEG and PEGylated small molecules remains laborious and unsatisfactory. In this report, we stably expressed a functional anti-PEG antibody on the surface of BALB 3T3 cells (3T3/alpha PEG cells) to develop a competitive enzyme-linked immunosorbent assay (ELISA) for PEG quantification. The alpha-PEG cell-coated plate bound biotinylated PEG(5K) (CH3-PEG(5K)-biotin) and CH3-PEG(5K)-I-131 more effectively than did a traditional anti-PEG antibody-coated plate. Competitive binding between PEG (2, 5, 10, or 20 kDa) and a known amount of CH3-PEG(5K)-biotin allowed construction of a reproducible competition curve. The alpha PEG cell-based competition ELISA measured small molecules derivatized by PEG(2K), PEG(5K), PEG(10K), PEG(20K), and PEG(5K) at concentrations as low as 58.6, 14.6, 3.7, 3.7, and 14.6 ng/mL respectively. Notably, the presence of serum or bovine serum albumin enhanced PEG measurement by the alpha PEG cell-based competition ELISA. Finally, we show here that the alpha PEG cell-based competition ELISA accurately delineated the pharmacokinetics of PEG(5K), comparable to those determined by direct measurement of radioactivity in blood after intravenous injection of CH3-PEG(5K)-I-131 into mice. This quantitative strategy may provide a simple and sensitive method for quantifying PEG and PEGylated small molecules in vivo.