Wallerian degeneration after crush injury of rat sciatic nerve increases endo- and epineurial tumor necrosis factor-alpha protein

被引：85

作者：

George, A ^{[1
]}

Buehl, A ^{[1
]}

Sommer, C ^{[1
]}

机构：

[1] Univ Wurzburg, Neurol Klin, D-8700 Wurzburg, Germany

来源：

NEUROSCIENCE LETTERS | 2004年 / 372卷 / 03期

关键词：

peripheral nerve injury; axonal damage; macrophages; neuropathic pain; cytokines; immunoassay;

D O I：

10.1016/j.neulet.2004.09.075

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The pro-inflammatory cytokine tumor necrosis factor-alpha (TNF) contributes to injury-induced peripheral nerve pathology and to the development of neuropathic pain. Here, we investigated whether TNF protein is altered at the site of crush injury of rat sciatic nerve using enzyme-linked immunoassay (ELISA) and immumohistochemistry (IHC). TNF protein levels determined by ELISA were low in nerve homogenates from naive rats. After crush injury, local TNF increased rapidly with a two-fold increase on day 0.5. TNF content remained elevated on day 3 and returned to baseline levels again by day 14 after crush. IHC revealed prominent TNF-immunoreactivity in many epineurial macrophages on days 0.5 to 3 after crush injury. These data indicate that TNF protein is early and transiently upregulated at the site of peripheral nerve trauma. (C) 2004 Elsevier Ireland Ltd. All rights reserved.

引用

页码：215 / 219

页数：5

共 16 条

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