The major acid soluble proteins of adult female Anopheles darlingi salivary glands include a member of the D7-related family of proteins

被引：12

作者：

Calvo, E

deBianchi, AG

James, AA

Marinotti, O ^{[1
]}

机构：

[1] Univ Sao Paulo, ICB, Dept Parasitol, BR-05508900 Sao Paulo, Brazil

[2] Univ Calif Irvine, Dept Mol Biol & Biochem, Irvine, CA 92697 USA

来源：

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY | 2002年 / 32卷 / 11期

基金：

巴西圣保罗研究基金会;

关键词：

Anopheles darlingi; mosquito; salivary glands; D7; protein; malaria vector;

D O I：

10.1016/S0965-1748(02)00062-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The salivary gland proteins of adult female Anopheles darlingi were fractionated by reverse-phase HPLC and the five major peaks were submitted for amino-terminal sequencing using automated Edman degradation. The amino acid sequence of one of the purified salivary gland proteins showed similarity with the D7r3 protein of An. gambiae. Cloning and sequencing of two cDNAs allowed the prediction of the complete sequence of the An. darlingi D7 protein. The D7r3 protein is present specifically in adult female salivary glands of An. darlingi and despite being one of the major salivary gland proteins its function is not known. Predictions of secondary and tertiary structures revealed the similarity of the An. darlingi D7 protein to insect odorant binding proteins. This suggests that D7 proteins may act as carriers of hydrophobic molecules in mosquito saliva. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：1419 / 1427

页数：9

共 32 条

[11] Characterization and cloning of a Tenebrio molitor hemolymph protein with sequence similarity to insect odorant-binding proteins [J].