A longitudinal magnetic resonance imaging (MRI) study of differences in abdominal fat distribution between normal mice, and lean overexpressors of mitochondrial uncoupling protein-3 (UCP-3)

被引:18
作者
Changani, KK [1 ]
Nicholson, A [1 ]
White, A [1 ]
Latcham, JK [1 ]
Reid, DG [1 ]
Clapham, JC [1 ]
机构
[1] GlaxoSmithKline, Welwyn Garden City AL6 9AR, Herts, England
关键词
uncoupling protein; UCP-3; MRI; fat; mitochondrial; distribution; mouse; lean; obesity;
D O I
10.1046/j.1463-1326.2003.00249.x
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Aim: To characterize evolution and distribution of abdominal adipose fat between 6 and 18 weeks of age in an animal model of energy consumption based on mice overexpressing the mitochondrial uncoupler protein 3 (UCP-3). Methods: T2-weighted multislice MRI was performed six times during the 12 week study; visceral, subcutaneous and intermuscular fat depots were quantified. Results: The overexpressor (UCP-3tg) mice consistently have less subcutaneous, visceral, interskeletal muscle and total fat throughout the experiment. Mean (standard error) volumes (ml) of the three distinct depots change between week 6 and week 18 as follows: wild type: subcutaneous 1.93 (0.28) to 6.18 (0.47), visceral 2.15 (0.34) to 6.37 (0.64), intermuscular 0.23 (0.04) to 0.53 (0.03); UCP-3tg: subcutaneous 1.47 (0.17) to 4.07 (0.57), visceral 1.18 (0.04) to 3.69 (0.59), intermuscular 0.23 (0.01) to 0.32 (0.04). Although they eat more (4.3 g compared with 3.4 g per day) the UCP-3tg's always weigh less than controls. In wild-type control animals, increases of all fat pools between week 6 and week 18 is highly significant, as it is for subcutaneous, visceral and total pools in the UCP-3tg animals. The UCP-3tg mice, however, show no significant absolute or relative increase in intermuscular fat; UCP-3 is predominantly overexpressed in skeletal muscle. Conclusion: MRI provides an excellent approach to comparative studies of fat distribution in animal models of energy expenditure such as the UCP-3tg mouse.
引用
收藏
页码:99 / 105
页数:7
相关论文
共 24 条
[1]   Use of proton MR spectroscopy and MR imaging to assess obesity [J].
BaracNieto, M ;
Gupta, RK .
JMRI-JOURNAL OF MAGNETIC RESONANCE IMAGING, 1996, 6 (01) :235-238
[2]   Non-invasive, quantitative assessment of the anatomical phenotype of corticotropin-releasing factor-overexpressing mice by MRI [J].
Beckmann, N ;
Gentsch, C ;
Baumann, D ;
Bruttel, K ;
Vassout, A ;
Schoeffter, P ;
Loetscher, E ;
Bobadilla, M ;
Perentes, E ;
Rudin, M .
NMR IN BIOMEDICINE, 2001, 14 (03) :210-216
[3]   Uncoupling protein-3: A new member of the mitochondrial carrier family with tissue-specific expression [J].
Boss, O ;
Samec, S ;
PaoloniGiacobino, A ;
Rossier, C ;
Dulloo, A ;
Seydoux, J ;
Muzzin, P ;
Giacobino, JP .
FEBS LETTERS, 1997, 408 (01) :39-42
[4]   The basal proton conductance of skeletal muscle mitochondria from transgenic mice overexpressing or lacking uncoupling protein-3 [J].
Cadenas, S ;
Echtay, KS ;
Harper, JA ;
Jekabsons, MB ;
Buckingham, JA ;
Grau, E ;
Abuin, A ;
Chapman, H ;
Clapham, JC ;
Brand, MD .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (04) :2773-2778
[5]   Mice overexpressing human uncoupling protein-3 in skeletal muscle are hyperphagic and lean [J].
Clapham, JC ;
Arch, JRS ;
Chapman, H ;
Haynes, A ;
Lister, C ;
Moore, GBT ;
Piercy, V ;
Carter, SA ;
Lehner, I ;
Smith, SA ;
Beeley, LJ ;
Godden, RJ ;
Herrity, N ;
Skehel, M ;
Changani, KK ;
Hockings, PD ;
Reid, DG ;
Squires, SM ;
Hatcher, J ;
Trail, B ;
Latcham, J ;
Rastan, S ;
Harper, AJ ;
Cadenas, S ;
Buckingham, JA ;
Brand, MD ;
Abuin, A .
NATURE, 2000, 406 (6794) :415-418
[6]   Superoxide activates mitochondrial uncoupling proteins [J].
Echtay, KS ;
Roussel, D ;
St-Pierre, J ;
Jekabsons, MB ;
Cadenas, S ;
Stuart, JA ;
Harper, JA ;
Roebuck, SJ ;
Morrison, A ;
Pickering, S ;
Clapham, JC ;
Brand, MD .
NATURE, 2002, 415 (6867) :96-99
[7]   CONTRIBUTION OF INTRAABDOMINAL FAT ACCUMULATION TO THE IMPAIRMENT OF GLUCOSE AND LIPID-METABOLISM IN HUMAN OBESITY [J].
FUJIOKA, S ;
MATSUZAWA, Y ;
TOKUNAGA, K ;
TARUI, S .
METABOLISM-CLINICAL AND EXPERIMENTAL, 1987, 36 (01) :54-59
[8]   Fast adipose tissue (FAT) assessment by MRI [J].
Gronemeyer, SA ;
Steen, RG ;
Kauffman, WM ;
Reddick, WE ;
Glass, JO .
MAGNETIC RESONANCE IMAGING, 2000, 18 (07) :815-818
[9]  
Inui A, 2000, PHARMACOL REV, V52, P35
[10]   WHICH IS THE PRIMARY ETIOLOGIC EVENT IN OTSUKA LONG-EVANS TOKUSHIMA FATTY RATS, A MODEL OF SPONTANEOUS NON-INSULIN-DEPENDENT DIABETES-MELLITUS, INSULIN-RESISTANCE, OR IMPAIRED INSULIN-SECRETION [J].
ISHIDA, K ;
MIZUNO, A ;
MIN, Z ;
SANO, T ;
SHIMA, K .
METABOLISM-CLINICAL AND EXPERIMENTAL, 1995, 44 (07) :940-945