Transmission and infectious dose of Escherichia coli O157:H7 in swine

被引:62
作者
Cornick, NA [1 ]
Helgerson, AF [1 ]
机构
[1] Iowa State Univ, Dept Vet Microbiol & Prevent Med, Ames, IA 50011 USA
关键词
D O I
10.1128/AEM.70.9.5331-5335.2004
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Escherichia coli O157:117 is only occasionally isolated from healthy swine, but some experimentally infected animals will shed the organism in their feces for at least 2 months. Potential explanations for the paucity of naturally occurring infections in swine, as compared to cattle, include a lack of animal-to-animal transmission so that the organism cannot be maintained within a herd, a high infectious dose, or herd management practices that prevent the maintenance of the organism in the gastrointestinal tract. We hypothesized that donor pigs infected with E. coli O157:117 would transmit the organism to naive pigs. We also determined the infectious dose and whether housing pigs individually on grated floors would decrease the magnitude or duration of fecal shedding. Infected donor pigs shedding <10(4) CFU of E. coli O157:117 per g transmitted the organism to 6 of 12 naive pigs exposed to them. The infectious dose of E. coli O157:117 for 3-month-old pigs was approximately 6 X 10(3) CFU. There was no difference in the magnitude and duration of fecal shedding by pigs housed individually on grates compared to those housed two per pen on cement floors. These results suggest that swine do not have an innate resistance to colonization by E. coli O157:H7 and that they could serve as a reservoir host under suitable conditions.
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页码:5331 / 5335
页数:5
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