Synthesis and enzymatic stability of phosphodiester-linked peptide - Oligonucleotide hybrids

被引：31

作者：

Robles, J ^{[1
]}

Maseda, M ^{[1
]}

Beltran, M ^{[1
]}

Concernau, M ^{[1
]}

Pedroso, E ^{[1
]}

Grandas, A ^{[1
]}

机构：

[1] UNIV BARCELONA,FAC QUIM,DEPT QUIM ORGAN,E-08028 BARCELONA,SPAIN

来源：

BIOCONJUGATE CHEMISTRY | 1997年 / 8卷 / 06期

关键词：

D O I：

10.1021/bc970051u

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Nucleopeptides Ac-Tyr(p(3')dACGT)-Ala-Phe-Gly-NH2, Ac-Thr(p(3')dACGT)-Ala-Phe-Gly-OH, Ac-Ser(p(3')dACGT)-Ala-Phe-Gly-OH, and Phac-Hse(p(3')dACGT)-Ala-Phe-Gly-OH, in which the 3'-end of a tetradeoxyribonucleotide is linked by a phosphodiester bond to a hydroxylated amino acid, were synthesized using a stepwise solid-phase methodology to study the influence of the linking amino acid on their stability to 3'-exonucleases. HPLC analysis of the reaction crudes after treatment of each nucleopeptide with snake venom phosphodiesterase showed that the lability of the amino acid-nucleoside linkage increases in the order Thr < Ser < Hse < Tyr.

引用

页码：785 / 788

页数：4

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