Preparative isolation and purification of hydroxyanthraquinones and cinnamic acid from the Chinese medicinal herb Rheum officinale Baill. by high-speed counter-current chromatography

被引：98

作者：

Liu, RM ^{[1
]}

Li, AF ^{[1
]}

Sun, AL ^{[1
]}

机构：

[1] Liaocheng Univ, Dept Chem & Chem Engn, Liaocheng 252059, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1052卷 / 1-2期

关键词：

Rheum officinale Baill; hydroxyanthraquinones; cinnamic acid; high-speed counter-current chromatography; pH-gradient elution;

D O I：

10.1016/j.chroma.2004.08.101

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-speed counter-current chromatography (HSCCC) method for preparative separation and purification of five hydroxyanthraquinones and cinnamic acid from the Chinese medicinal herb Rheum officinale Baill. was developed by using pH-gradient elution. The purities of them, emodin, aloe-emodin, chrysophanol, physcion and cinnamic acid were all over 98%, as determined by high performance liquid chromatography (HPLC). The structures of them were identified by H-1 NMR. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：217 / 221

页数：5

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