Characterization of fibrinogen adsorption onto glass microcapillary surfaces by ELISA

被引:27
作者
Salim, Malinda
O'Sullivan, Brian
McArthur, Sally L.
Wright, Phillip C.
机构
[1] Univ Sheffield, Dept Chem & Proc Engn, Sheffield S1 3JD, S Yorkshire, England
[2] Univ Sheffield, Dept Mat Engn, Sheffield S3 7HQ, S Yorkshire, England
基金
英国工程与自然科学研究理事会;
关键词
D O I
10.1039/b612521m
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Adsorption of biomolecules onto microchannel surfaces remains a critical issue in microfluidic devices. This paper investigates the adsorption of fibrinogen on glass microcapillaries using an immunoassay method (ELISA) and X-ray photoelectron spectroscopy (XPS). Various adsorption conditions such as protein concentrations and incubation times, buffer pH, buffer ionic strengths and effects of flow are presented. ELISA is successfully demonstrated as a facile and robust technique to examine these phenomena. The highest adsorption level occurs near the isoelectric point of fibrinogen (pH 5.0) and low buffer ionic strengths (0-8 mM). Microchannel surface saturation was achieved at a fibrinogen solution concentration of similar to 50 mu g ml(-1). Fibrinogen adsorption under flow was always higher than that seen in static systems. The importance of diffusion phenomena in microchannels on protein adsorption was demonstrated. ELISA experiments using fused silica and PEEK have also confirmed significant adsorption on these mass spectrometer transfer line materials.
引用
收藏
页码:64 / 70
页数:7
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