Planar cell polarity-mediated induction of neural stem cell expansion during axolotl spinal cord regeneration

被引：56

作者：

Albors, Aida Rodrigo ^{[1
,2
,3
]}

Tazaki, Akira ^{[1
,2
,3
]}

Rost, Fabian ^{[4
]}

Nowoshilow, Sergej ^{[1
,2
,3
]}

Chara, Osvaldo ^{[4
,5
]}

Tanaka, Elly M. ^{[1
,2
,3
]}

机构：

[1] Deutsch Forsch Gemeinschaft, Ctr Regenerat Therapies Dresden, Dresden, Germany

[2] Max Planck Inst Mol Cell Biol & Genet, Dresden, Germany

[3] Tech Univ Dresden, D-01062 Dresden, Germany

[4] Tech Univ Dresden, Ctr Informat Serv & High Performance Comp, D-01062 Dresden, Germany

[5] Univ Nacl La Plata, Natl Sci & Tech Res Council, Inst Phys Liquids & Biol Syst, RA-1900 La Plata, Buenos Aires, Argentina

来源：

ELIFE | 2015年 / 4卷

关键词：

MITOTIC SPINDLE ORIENTATION; CONVERGENT EXTENSION; DIVISION ORIENTATION; GLIAL-CELLS; GENE; GROWTH; EXPRESSION; TAIL; WNT; PROLIFERATION;

D O I：

10.7554/eLife.10230

中图分类号：

Q [生物科学];

学科分类号：

090105 [作物生产系统与生态工程];

摘要：

Axolotls are uniquely able to mobilize neural stem cells to regenerate all missing regions of the spinal cord. How a neural stem cell under homeostasis converts after injury to a highly regenerative cell remains unknown. Here, we show that during regeneration, axolotl neural stem cells repress neurogenic genes and reactivate a transcriptional program similar to embryonic neuroepithelial cells. This dedifferentiation includes the acquisition of rapid cell cycles, the switch from neurogenic to proliferative divisions, and the re-expression of planar cell polarity (PCP) pathway components. We show that PCP induction is essential to reorient mitotic spindles along the anterior-posterior axis of elongation, and orthogonal to the cell apical-basal axis. Disruption of this property results in premature neurogenesis and halts regeneration. Our findings reveal a key role for PCP in coordinating the morphogenesis of spinal cord outgrowth with the switch from a homeostatic to a regenerative stem cell that restores missing tissue.

引用

页数：29

共 102 条

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