Boron dipyrromethene (BODIPY) functionalized carbon nano-onions for high resolution cellular imaging

被引：65

作者：

Bartelmess, Juergen ^{[1
]}

De Luca, Elisa ^{[2
]}

Signorelli, Angelo ^{[1
]}

Baldrighi, Michele ^{[1
]}

Becce, Michele ^{[1
]}

Brescia, Rosaria ^{[3
]}

Nardone, Valentina ^{[4
,5
]}

Parisini, Emilio ^{[4
]}

Echegoyen, Luis ^{[6
]}

Pompa, Pier Paolo ^{[2
]}

Giordani, Silvia ^{[1
]}

机构：

[1] IIT, Nanophys Dept, I-16163 Genoa, Italy

[2] IIT, Ctr Biomol Nanotechnol, I-73010 Lecce, Italy

[3] IIT, Nanochem Dept, I-16163 Genoa, Italy

[4] IIT, CNST, I-20133 Milan, Italy

[5] Politecn Milan, Dept Chem Mat & Chem Engn Giulio Natta, I-20131 Milan, Italy

[6] Univ Texas El Paso, Dept Chem, El Paso, TX 79968 USA

来源：

NANOSCALE | 2014年 / 6卷 / 22期

基金：

美国国家科学基金会;

关键词：

ENERGY-TRANSFER; NANOTUBES; DYES; FLUORESCENT; DELIVERY; DRUG; PORPHYRIN; GRAPHENE; ELECTRON; SYSTEM;

D O I：

10.1039/c4nr04533e

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

Carbon nano-onions (CNOs) are an exciting class of carbon nanomaterials, which have recently demonstrated a facile cell-penetration capability. In the present work, highly fluorescent boron dipyrromethene (BODIPY) dyes were covalently attached to the surface of CNOs. The introduction of this new carbon nanomaterial-based imaging platform, made of CNOs and BODIPY fluorophores, allows for the exploration of synergetic effects between the two building blocks and for the elucidation of its performance in biological applications. The high fluorescence intensity exhibited by the functionalized CNOs translates into an excellent in vitro probe for the high resolution imaging of MCF-7 human breast cancer cells. It was also found that the CNOs, internalized by the cells by endocytosis, localized in the lysosomes and did not show any cytotoxic effects. The presented results highlight CNOs as excellent platforms for biological and biomedical studies due to their low toxicity, efficient cellular uptake and low fluorescence quenching of attached probes.

引用

页码：13761 / 13769

页数：9

共 60 条

[1]

Boron dipyrromethene (BODIPY)-based photosensitizers for photodynamic therapy [J].