A novel putrescine utilization pathway involves γ-glutamylated intermediates of Escherichia coli K-12

被引：140

作者：

Kurihara, S ^{[1
]}

Oda, S ^{[1
]}

Kato, K ^{[1
]}

Kim, HG ^{[1
]}

Koyanagi, T ^{[1
]}

Kumagai, H ^{[1
]}

Suzuki, H ^{[1
]}

机构：

[1] Kyoto Univ, Div Integrated Life Sci, Grad Sch Biostudies, Sakyo Ku, Kyoto 6068502, Japan

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2005年 / 280卷 / 06期

关键词：

D O I：

10.1074/jbc.M411114200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel bacterial putrescine utilization pathway was discovered. Seven genes, the functions of whose products were not known, are involved in this novel pathway. Five of them encode enzymes that catabolize putrescine; one encodes a putrescine importer, and the other encodes a transcriptional regulator. This novel pathway involves six sequential steps as follows: 1) import of putrescine; 2) ATP-dependent gamma-glutamylation of putrescine; 3) oxidization of gamma-glutamylputrescine; 4) dehydrogenation of gamma-glutamyl-gamma-aminobutyraldehyde; 5) hydrolysis of the gamma-glutamyl linkage of gamma-glutamyl-gamma-aminobutyrate; and 6) transamination of gamma-aminobutyrate to form the final product of this pathway, succinate semialdehyde, which is the precursor of succinate.

引用

页码：4602 / 4608

页数：7

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