Sensitive assay of RNA interference in Drosophila and Chinese hamster cultured cells using firefly luciferase gene as target

被引：84

作者：

Ui-Tei, K

Zenno, SH

Miyata, Y

Saigo, K

机构：

[1] Univ Tokyo, Grad Sch Sci, Dept Biophys & Biochem, Bunkyo Ku, Tokyo 1130033, Japan

[2] Nippon Med Sch, Dept Pharmacol, Bunkyo Ku, Tokyo 1138602, Japan

来源：

FEBS LETTERS | 2000年 / 479卷 / 03期

关键词：

RNA interference; double-stranded RNA; Chinese hamster; cultured cell; Drosophila;

D O I：

10.1016/S0014-5793(00)01883-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive cellular assay system for RNA interference was developed using the firefly luciferase gene as target. RNA interference was noted not only in Drosophila cultured cells but Chinese hamster cells (CHO-KI) as well, although double-stranded RNA required for the latter was 2500 times more than for the former. Cognate double-stranded RNA as short as 38 bp was found to be still capable of inducing RNA interference in Drosophila cultured cells. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：79 / 82

页数：4

共 24 条

[1] Double-stranded RNA specifically disrupts gene expression during planarian regeneration [J].