The Sonogel-Carbon materials as basis for development of enzyme biosensors for phenols and polyphenols monitoring: A detailed comparative study of three immobilization matrixes

被引:37
作者
El Kaoutit, Mohammed
Naranjo-Rodriguez, Ignacio
Temsamani, Khalid Riffi
Hidalgo-Hidalgo de Cisneros, Jose Luis
机构
[1] Univ Cadiz, Fac Ciencias, Dept Quim Analit, E-11510 Puerto Real, Cadiz, Spain
[2] Univ Abdelmalek Essaadi, Fac Sci Tetouan, Equipe Rech Electrochim & Syst Interac, Dept Chim, M Hannech II 93002, Morocco
关键词
biosensors; sonogel-carbon; tyrosinase; phenols; polyphenols; amperometry;
D O I
10.1016/j.bios.2006.12.008
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Three amperometric biosensors based on immobilization of tyrosinase on a new Sonogel-Carbon electrode for detection of phenols and polyphenols are described. The electrode was prepared using high energy ultrasounds (HEU) directly applied to the precursors. The first biosensor was obtained by simple adsorption of the enzyme on the Sonogel-Carbon electrode. The second and the third ones, presenting sandwich configurations, were initially prepared by adsorption of the enzyme and then modification by mean of polymeric membrane such as polyethylene glycol for the second one, and the ion-exchanger Nafion in the case of the third biosensor. The optimal enzyme loading and polymer concentration, in the second layer, were found to be 285 U and 0.5%, respectively. All biosensors showed optimal activity at the following conditions: pH 7, -200 mV, and 0.02 mol l(-1) phosphate buffer. The response of the biosensors toward five simple phenols derivatives and two polyphenols were investigated. It was found that the three developed tyrosinase Sonogel-Carbon based biosensors are in satisfactory competitiveness for phenolic compounds determination with other tyrosinase based biosensors reported in the literature. The detection limit, sensitivity, and the apparent Michaelis-Menten constant KM for the Nafion modified biosensor were, respectively, 0.064, 0.096, and 0.03 mu mol, 82.5, 63.4, and 194 nA mu mol(-1) 1(-1), and 67.1, 54.6, and 12.1 mu mol 1(-1) for catechol, phenol, and 4-chloro-3-methylphenol. Hill coefficient values (around 1 for all cases), demonstrated that the immobitization method does not affect the nature of the enzyme and confirms the biocompatibility of the Sonogel-Carbon with the bioprobe. An exploratory application to real samples such as beers, river waters and tannery wastewaters showed the ability of the developed Nafion/tyrosinase/Sonogel-Carbon biosensor to retain its stable and reproducible response. (c) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码:2958 / 2966
页数:9
相关论文
共 46 条
[1]   Synthesis and characterization of a pyrrole-alginate conjugate and its application in a biosensor construction [J].
Abu-Rabeah, K ;
Polyak, B ;
Ionescu, RE ;
Cosnier, S ;
Marks, RS .
BIOMACROMOLECULES, 2005, 6 (06) :3313-3318
[2]   Synthesis and electrochemical characterisation of novel sonogel-carbon-polythiophene microstructured electrodes [J].
Ballarin, B ;
Zanardi, C ;
Schenetti, L ;
Seeber, R ;
de Cisneros, JLHH .
SYNTHETIC METALS, 2003, 139 (01) :29-33
[3]   Electrocatalytic activity of cobalt phthalocyanine stabilized by different matrixes [J].
Ballarin, B ;
Gazzano, M ;
de Cisneros, JLHH ;
Tonelli, D ;
Seeber, R .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2002, 374 (05) :891-897
[4]   Amperometric flow-injection determination of phenolic compounds at self-assembled monolayer-based tyrosinase biosensors [J].
Campuzano, S ;
Serra, B ;
Pedrero, M ;
de Villena, FJM ;
Pingarrón, JM .
ANALYTICA CHIMICA ACTA, 2003, 494 (1-2) :187-197
[5]   Sol-gels and electrochemistry: Research at the intersection [J].
Collinson, MM ;
Howells, AR .
ANALYTICAL CHEMISTRY, 2000, 72 (21) :702A-709A
[6]   Study of the responses of a sonogel-carbon electrode towards phenolic compounds [J].
Cordero-Rando, MD ;
Naranjo-Rodríguez, I ;
Palacios-Santander, JM ;
Cubillana-Aguilera, LM ;
Hidalgo-Hidalgo-de-Cisneros, JL .
ELECTROANALYSIS, 2005, 17 (09) :806-814
[7]   The Sonogel-Carbon electrode as a sol-gel graphite-based electrode [J].
Cordero-Rando, MDM ;
de Cisneros, JLHH ;
Blanco, E ;
Naranjo-Rodríguez, I .
ANALYTICAL CHEMISTRY, 2002, 74 (10) :2423-2427
[8]   Development of a PPO-poly(amphiphilic pyrrole) electrode for on site monitoring of phenol in aqueous effluents [J].
Cosnier, S ;
Fombon, JJ ;
Labbé, P ;
Limosin, D .
SENSORS AND ACTUATORS B-CHEMICAL, 1999, 59 (2-3) :134-139
[9]   An bienzyme electrode (alkaline phosphatase polyphenol oxidase) for the amperometric determination of phosphate [J].
Cosnier, S ;
Gondran, C ;
Watelet, JC ;
De Giovani, W ;
Furriel, RPM ;
Leone, FA .
ANALYTICAL CHEMISTRY, 1998, 70 (18) :3952-3956
[10]   A NEW STRATEGY FOR THE CONSTRUCTION OF A TYROSINASE-BASED AMPEROMETRIC PHENOL AND O-DIPHENOL SENSOR [J].
COSNIER, S ;
INNOCENT, C .
BIOELECTROCHEMISTRY AND BIOENERGETICS, 1993, 31 (02) :147-160