The minimal cadherin-catenin complex binds to actin filaments under force

被引：454

作者：

Buckley, Craig D. ^{[1
]}

Tan, Jiongyi ^{[2
]}

Anderson, Karen L. ^{[3
]}

Hanein, Dorit ^{[3
]}

Volkmann, Niels ^{[3
]}

Weis, William I. ^{[2
,4
,5
]}

Nelson, W. James ^{[5
,6
]}

Dunn, Alexander R. ^{[1
,2
,7
]}

机构：

[1] Stanford Univ, Dept Chem Engn, Stanford, CA 94305 USA

[2] Stanford Univ, Biophys Program, Stanford, CA 94305 USA

[3] Sanford Burnham Med Res Inst, Bioinformat & Struct Syst Biol Program, La Jolla, CA 92037 USA

[4] Stanford Univ, Dept Biol Struct, Stanford, CA 94305 USA

[5] Stanford Univ, Dept Mol & Cellular Physiol, Stanford, CA 94305 USA

[6] Stanford Univ, Dept Biol, Stanford, CA 94305 USA

[7] Stanford Univ, Stanford Cardiovascular Inst, Stanford, CA 94305 USA

来源：

SCIENCE | 2014年 / 346卷 / 6209期

关键词：

CELL-ADHESION MOLECULES; ALPHA-E-CATENIN; BETA-CATENIN; CATCH BONDS; ADHERENS JUNCTIONS; F-ACTIN; SLIP BONDS; VINCULIN; PROTEIN; DISSOCIATION;

D O I：

10.1126/science.1254211

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Linkage between the adherens junction (AJ) and the actin cytoskeleton is required for tissue development and homeostasis. In vivo findings indicated that the AJ proteins E-cadherin, beta-catenin, and the filamentous (F)-actin binding protein alpha E-catenin form a minimal cadherin-catenin complex that binds directly to F-actin. Biochemical studies challenged this model because the purified cadherin-catenin complex does not bind F-actin in solution. Here, we reconciled this difference. Using an optical trap-based assay, we showed that the minimal cadherin-catenin complex formed stable bonds with an actin filament under force. Bond dissociation kinetics can be explained by a catch-bond model in which force shifts the bond from a weakly to a strongly bound state. These results may explain how the cadherin-catenin complex transduces mechanical forces at cell-cell junctions.

引用

页码：600 / +

页数：9

共 52 条

[1]

EPLIN mediates linkage of the cadherin-catenin complex to F-actin and stabilizes the circumferential actin belt [J].

Abe, Kentaro ;

Takeichi, Masatoshi .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (01) :13-19

[2]

α-Catenin cytomechanics - role in cadherin-dependent adhesion and mechanotransduction [J].

Barry, Adrienne K. ;

Tabdili, Hamid ;

Muhamed, Ismaeel ;

Wu, Jun ;

Shashikanth, Nitesh ;

Gomez, Guillermo A. ;

Yap, Alpha S. ;

Gottardi, Cara J. ;

de Rooij, Johan ;

Wang, Ning ;

Leckband, Deborah E. .

JOURNAL OF CELL SCIENCE, 2014, 127 (08) :1779-1791

[3]

Dynamics of unbinding of cell adhesion molecules: Transition from catch to slip bonds [J].

Barsegov, V ;

Thirumalai, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (06) :1835-1839

[4]

Dynamic response of adhesion complexes: Beyond the single-path picture [J].

Bartolo, Denis ;

Derényi, Imre ;

Ajdari, Armand .

Physical Review E - Statistical, Nonlinear, and Soft Matter Physics, 2002, 65 (05) :1-051910

[5]

BELL GI, 1978, SCIENCE, V200, P618, DOI 10.1126/science.347575

[6]

E-cadherin is under constitutive actomyosin-generated tension that is increased at cell-cell contacts upon externally applied stretch [J].

Borghi, Nicolas ;

Sorokina, Maria ;

Shcherbakova, Olga G. ;

Weis, William I. ;

Pruitt, Beth L. ;

Nelson, W. James ;

Dunn, Alexander R. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (31) :12568-12573

[7]

Mechanical Feedback through E-Cadherin Promotes Direction Sensing during Collective Cell Migration [J].

Cai, Danfeng ;

Chen, Shann-Ching ;

Prasad, Mohit ;

He, Li ;

Wang, Xiaobo ;

Choesmel-Cadamuro, Valerie ;

Sawyer, Jessica K. ;

Danuser, Gaudenz ;

Montell, Denise J. .

CELL, 2014, 157 (05) :1146-1159

[8]

Plasticity of hydrogen bond networks regulates mechanochemistry of cell adhesion complexes [J].

Chakrabarti, Shaon ;

Hinczewski, Michael ;

Thirumalai, D. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2014, 111 (25) :9048-9053

[9]

αE-catenin is an autoinhibited molecule that coactivates vinculin [J].

Choi, Hee-Jung ;

Pokutta, Sabine ;

Cadwell, Gregory W. ;

Bobkov, Andrey A. ;

Bankston, Laurie A. ;

Liddington, Robert C. ;

Weis, William I. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (22) :8576-8581

[10]

Loop 2 of myosin is a force-dependent inhibitor of the rigor bond [J].

Clobes, Amy M. ;

Guilford, William H. .

JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY, 2014, 35 (02) :143-152

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