Characterisation of the high-molecular weight fructan isolated from garlic (Allium sativum L.)

被引：86

作者：

Baumgartner, S

Dax, TG

Praznik, W

Falk, H

机构：

[1] IFA Tulln, Inst Agrobiotechnol, Ctr Analyt Chem, A-3430 Tulln, Austria

[2] Agr Univ Vienna, Inst Chem, A-1190 Vienna, Austria

[3] Johannes Kepler Univ, Inst Chem, A-4040 Linz, Austria

来源：

CARBOHYDRATE RESEARCH | 2000年 / 328卷 / 02期

基金：

奥地利科学基金会;

关键词：

fructan; NMR; reductive cleavage; endo-inulinase;

D O I：

10.1016/S0008-6215(00)00097-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A high molecular weight fructan was isolated from garlic and the structure determined by enzymatic, chemical and spectroscopic (NMR) methods. It was found that the garlic fructan belongs to the neokestose family. It has a (2 --> 1)-linked beta-D-Fruf backbone with (2 --> 6)-linked beta-D-Fruf side chains. A structural model was postulated for a degree of polymerisation of about 58. This model was substantiated using an endo-inulinase purified from Aspergillus ficuum and by H-1 and C-13 NMR spectroscopy. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：177 / 183

页数：7

共 25 条

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