Flow of microgel capsules through topographically patterned microchannels

We investigated the flow dynamics of microgel capsules in topographically patterned microfluidic devices. For microgels flowing through channel constrictions, or orifices, we observed three phenomena: ( i) the effect of confinement, ( ii) the role of interactions between the microgels and the channel surface, and ( iii) the effect of the velocities of microgels prior to their passage through an orifice. We studied negatively charged alginate microgels and positively charged alginate microgels coated with N-(2-hydroxy) propyl-3-trimethylammonium chitosan chloride ( HTCC). Aqueous dispersions of microgels were driven through poly( dimethyl siloxane) microchannels carrying a weak negative surface charge. The velocity of the continuous phase, and hence, the velocity of the microgels increased as they passed through topographically patterned orifices. Alginate microgels were observed to have a larger increase in velocity relative to HTCC-coated alginate microgels. This effect, which was attributed to electrostatic attraction or repulsion, was found to be strongest for orifices with dimensions close to the microgel diameter. For example, when 75 mu m-diameter microgels flowed through a 76 mu m orifice, alginate gels ( negatively charged) experienced a 26 greater increase in velocity than HTCC- coated ( postitively charged) microgels. This effect was exaggerated at lower initial flow rates. For example, when 75 mu m-diameter microgels flowed through an 80 mm orifice, a two-fold difference in the velocity changes of the two microgel types was observed when the initial flow rate was 275 mu m s(-1), while a three- fold difference in velocity changes was observed when the initial flow rate was 130 mu m s(-1). We speculate that these studies will be useful for modeling the flow of suspensions of cells or other biologically relevant particles for a wide range of applications.