Low effectiveness of DNA vaccination against HER-2/neu in ageing

被引：33

作者：

Provinciali, M

Smorlesi, A

Donnini, A

Bartozzi, B

Amici, A

机构：

[1] INRCA, Lab Tumor Immunol, Gerontol Res Dept, Ctr Immunol, I-60121 Ancona, Italy

[2] Univ Camerino, Dept Biol Mol Cellular & Anim, I-62032 Camerino, Italy

来源：

VACCINE | 2003年 / 21卷 / 9-10期

关键词：

HER-2/neu; DNA vaccination; ageing;

D O I：

10.1016/S0264-410X(02)00530-3

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We evaluated the effectiveness of vaccination with a HER-2/neu DNA plasmid to induce protective immunity against HER-2/neu overexpressing syngeneic TUBO tumour cells in old ages. Young and old Balb/c mice received three immunizations with a pCMVneuNT DNA plasmid and, successively, were challenged with TUBO cells. Young mice were completely protected whereas less than 60% protection was observed in old mice. Anti-p185(neu) antibodies were found in the sera from both young and old immunized mice, even if antibody production was significantly higher in young in comparison with old mice. Similarly, higher anti-p185(neu), lymphocyte proliferation was induced in young than in old mice. No anti-p185(neu) cytotoxicity was found in lymphocytes from old animals. We conclude that anticancer DNA vaccination has a lower effectiveness in old than in young ages. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：843 / 848

页数：6

共 18 条

[1] DNA vaccination with full-length or truncated Neu induces protective immunity against the development of spontaneous mammary tumors in HER-2/neu transgenic mice [J].