A Universal Live Cell Barcoding-Platform for Multiplexed Human Single Cell Analysis

被引：61

作者：

Hartmann, Felix J. ^{[1
]}

Simonds, Erin F. ^{[2
]}

Bendall, Sean C. ^{[1
]}

机构：

[1] Stanford Univ, Sch Med, Dept Pathol, Palo Alto, CA 94304 USA

[2] UCSF, Dept Neurol, San Francisco, CA USA

来源：

SCIENTIFIC REPORTS | 2018年 / 8卷

基金：

瑞士国家科学基金会;

关键词：

HIGH-DIMENSIONAL CYTOMETRY; MASS-CYTOMETRY; ANALYSIS REVEALS; CLASS-I; IMMUNE; PROGRESSION; EXPRESSION; CISPLATIN; VIRUS;

D O I：

10.1038/s41598-018-28791-2

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Single-cell barcoding enables the combined processing and acquisition of multiple individual samples as one. This maximizes assay efficiency and eliminates technical variability in both sample preparation and analysis. Remaining challenges are the barcoding of live, unprocessed cells to increase downstream assay performance combined with the flexibility of the approach towards a broad range of cell types. To that end, we developed a novel antibody-based platform that allows the robust barcoding of live human cells for mass cytometry (CyTOF). By targeting both the MHC class I complex (beta-2-microglobulin) and a broadly expressed sodium-potassium ATPase-subunit (CD298) with platinum-conjugated antibodies, human immune cells, stem cells as well as tumor cells could be multiplexed in the same single-cell assay. In addition, we present a novel palladium-based covalent viability reagent compatible with this barcoding strategy. Altogether, this platform enables mass cytometry-based, live-cell barcoding across a multitude of human sample types and provides a scheme for multiplexed barcoding of human singlecell assays in general.

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页数：10

相关论文

共 46 条

[1]

The road ahead: Implementing mass cytometry in clinical studies, one cell at a time [J].

Baca, Quentin ;

Cosma, Antonio ;

Nolan, Garry ;

Gaudilliere, Brice .

CYTOMETRY PART B-CLINICAL CYTOMETRY, 2017, 92 (01) :10-11

[2]

A Mass Spectrometric-Derived Cell Surface Protein Atlas [J].

Bausch-Fluck, Damaris ;

Hofmann, Andreas ;

Bock, Thomas ;

Frei, Andreas P. ;

Cerciello, Ferdinando ;

Jacobs, Andrea ;

Moest, Hansjoerg ;

Omasit, Ulrich ;

Gundry, Rebekah L. ;

Yoon, Charles ;

Schiess, Ralph ;

Schmidt, Alexander ;

Mirkowska, Paulina ;

Haertlova, Anetta ;

Van Eyk, Jennifer E. ;

Bourquin, Jean-Pierre ;

Aebersold, Ruedi ;

Boheler, Kenneth R. ;

Zandstra, Peter ;

Wollscheid, Bernd .

PLOS ONE, 2015, 10 (04)

[3]

Single-Cell Trajectory Detection Uncovers Progression and Regulatory Coordination in Human B Cell Development [J].

Bendall, Sean C. ;

Davis, Kara L. ;

Amir, El-ad David ;

Tadmor, Michelle D. ;

Simonds, Erin F. ;

Chen, Tiffany J. ;

Shenfeld, Daniel K. ;

Nolan, Garry P. ;

Pe'er, Dana .

CELL, 2014, 157 (03) :714-725

[4]

Single-Cell Mass Cytometry of Differential Immune and Drug Responses Across a Human Hematopoietic Continuum [J].

Bendall, Sean C. ;

Simonds, Erin F. ;

Qiu, Peng ;

Amir, El-ad D. ;

Krutzik, Peter O. ;

Finck, Rachel ;

Bruggner, Robert V. ;

Melamed, Rachel ;

Trejo, Angelica ;

Ornatsky, Olga I. ;

Balderas, Robert S. ;

Plevritis, Sylvia K. ;

Sachs, Karen ;

Pe'er, Dana ;

Tanner, Scott D. ;

Nolan, Garry P. .

SCIENCE, 2011, 332 (6030) :687-696

[5]

Multiplexed mass cytometry profiling of cellular states perturbed by small-molecule regulators [J].

Bodenmiller, Bernd ;

Zunder, Eli R. ;

Finck, Rachel ;

Chen, Tiffany J. ;

Savig, Erica S. ;

Bruggner, Robert V. ;

Simonds, Erin F. ;

Bendall, Sean C. ;

Sachs, Karen ;

Krutzik, Peter O. ;

Nolan, Garry P. .

NATURE BIOTECHNOLOGY, 2012, 30 (09) :858-U89

[6]

Bubeník J, 2004, INT J ONCOL, V25, P487

[7]

Algorithmic Tools for Mining High-Dimensional Cytometry Data [J].

Chester, Cariad ;

Maecker, Holden T. .

JOURNAL OF IMMUNOLOGY, 2015, 195 (03) :773-779

[8]

A platinum-based covalent viability reagent for single-cell mass cytometry [J].

Fienberg, Harris G. ;

Simonds, Erin F. ;

Fantl, Wendy J. ;

Nolan, Garry P. ;

Bodenmiller, Bernd .

CYTOMETRY PART A, 2012, 81A (06) :467-475

[9]

Normalization of mass cytometry data with bead standards [J].

Finck, Rachel ;

Simonds, Erin F. ;

Jager, Astraea ;

Krishnaswamy, Smita ;

Sachs, Karen ;

Fantl, Wendy ;

Pe'er, Dana ;

Nolan, Garry P. ;

Bendall, Sean C. .

CYTOMETRY PART A, 2013, 83A (05) :483-+

[10]

Say No to DMSO: Dimethylsulfoxide Inactivates Cisplatin, Carboplatin, and Other Platinum Complexes [J].

Hall, Matthew D. ;

Telma, Katherine A. ;

Chang, Ki-Eun ;

Lee, Tobie D. ;

Madigan, James P. ;

Lloyd, John R. ;

Goldlust, Ian S. ;

Hoeschele, James D. ;

Gottesman, Michael M. .

CANCER RESEARCH, 2014, 74 (14) :3913-3922

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