Topological isomers of human uroguanylin: Interconversion between biologically active and inactive isomers

被引：13

作者：

Chino, N

Kubo, S

Kitani, T

Yoshida, T

Tanabe, R

Kobayashi, Y

Nakazato, M

Kangawa, K

Kimura, T

机构：

[1] Peptide Inst Inc, Prot Res Fdn, Osaka 562, Japan

[2] Osaka Univ, Fac Pharmaceut Sci, Osaka 565, Japan

[3] Miyazaki Med Coll, Dept Internal Med 3, Miyazaki 88916, Japan

[4] Natl Cardiovasc Ctr, Res Inst, Osaka 565, Japan

来源：

FEBS LETTERS | 1998年 / 421卷 / 01期

关键词：

uroguanylin; guanylin; topological isomer; interconversion; HPLC analysis; biological activity;

D O I：

10.1016/S0014-5793(97)01527-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The solution structures of the two compounds of human uroguanylin (I and II), which were generated during disulfide bond forming reaction, were found to be topological isomers by H-1-nuclear magnetic resonance spectroscopy. These isomers are interconvertible in aqueous media at rates which vary with the pH and temperature of the solution, Because compound I is active in the cGMP producing assay, but compound II is not, this interconversion may be useful for evaluating the activity of human uroguanylin both in vivo and in vitro., (C) 1998 Federation of European Biochemical Societies.

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页码：27 / 31

页数：5

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