Hydrolysis of DNA and its molecular components in the dry state

被引：15

作者：

Marrone, April ^{[1
,2
,3
]}

Ballantyne, Jack ^{[1
,2
,3
]}

机构：

[1] Natl Ctr Forens Sci, Orlando, FL 32816 USA

[2] Univ Cent Florida, Dept Chem, Orlando, FL 32816 USA

[3] Univ Cent Florida, Grad Program Chem, Orlando, FL 32816 USA

来源：

FORENSIC SCIENCE INTERNATIONAL-GENETICS | 2010年 / 4卷 / 03期

关键词：

Hydrolysis of DNA; Dry state DNA; Depurination; PERFORMANCE LIQUID-CHROMATOGRAPHY; ACID-CATALYZED HYDROLYSIS; DEOXYRIBONUCLEIC-ACID; ABASIC SITE; CYTOSINE RESIDUES; DEAMINATION; MECHANISM; HEAT; PYRIMIDINES; DERIVATIVES;

D O I：

10.1016/j.fsigen.2009.08.007

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The biochemistry of dry state DNA is of interest to the field of forensic biology. The precise chemical nature of the hydrolytic degradation products of the DNA molecule in the dry state has not been previously investigated in detail. In this study we found that the mechanistic chemistry of DNA hydrolysis appears to be the same for the hydrated and dry states. The thermodynamic parameters are also similar in both states and the activation energies for base hydrolysis are indistinguishable. The principal difference between the two states is the rate at which hydrolytic degradation occurs. The duplex configuration of dry state DNA offers much more protection for the molecule than is offered in single strand and nucleotide species. Single strand breaks of dry state duplex DNA occur with a half life of 24 +/- 2 days at 65 degrees C and appear to occur in a mechanistic manner. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

引用

页码：168 / 177

页数：10

共 31 条

[1] Assessment of DHPLC usefulness in the genotyping of GSTP1 exon 5 SNP: comparison to the PCR-RFLP method [J].