An Improved Protocol for the Preparation of Total Genomic DNA from Isolates of Yeast and Mould Using Whatman FTA Filter Papers

被引：24

作者：

Borman, Andrew M. ^{[1
,2
]}

Fraser, Mark ^{[2
]}

Linton, Christopher J. ^{[2
]}

Palmer, Michael D. ^{[2
]}

Johnson, Elizabeth M. ^{[2
]}

机构：

[1] HPA Mycol Reference Lab, Bristol BS2 8EL, Avon, England

[2] Hlth Protect Agcy, SW Reg Lab, Mycol Reference Lab, Bristol, Avon, England

来源：

MYCOPATHOLOGIA | 2010年 / 169卷 / 06期

关键词：

Fungal genomic DNA; FTA technology; PCR; DNA extraction; Molecular identification; SPECIES COMPLEX; PSEUDALLESCHERIA; FUSARIUM;

D O I：

10.1007/s11046-010-9284-7

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Here, we present a significantly improved version of our previously published method for the extraction of fungal genomic DNA from pure cultures using Whatman FTA(R) filter paper matrix technology. This modified protocol is extremely rapid, significantly more cost effective than our original method, and importantly, substantially reduces the problem of potential cross-contamination between sequential filters when employing FTA technology.

引用

页码：445 / 449

页数：5