CREB expression in the brains of two closely related parasitic wasp species that differ in long-term memory formation

被引：13

作者：

van den Berg, M. ^{[1
]}

Verbaarschot, P. ^{[1
]}

Hontelez, S. ^{[1
]}

Vet, L. E. M. ^{[1
,2
]}

Dicke, M. ^{[1
]}

Smid, H. M. ^{[1
]}

机构：

[1] Wageningen Univ, Entomol Lab, NL-6700 EH Wageningen, Netherlands

[2] Netherlands Inst Ecol NIOO KNAW, Dept Multitroph Interact, Heteren, Netherlands

来源：

INSECT MOLECULAR BIOLOGY | 2010年 / 19卷 / 03期

关键词：

learning; insect; parasitoid wasp; Cotesia glomerata; Cotesia rubecula; ELEMENT-BINDING-PROTEIN; TRANSCRIPTION FACTOR CREB; CENTRAL-NERVOUS-SYSTEM; CYCLIC-AMP; MESSENGER-RNA; COTESIA-GLOMERATA; TARGETED MUTATION; CAMP; DROSOPHILA; ACTIVATOR;

D O I：

10.1111/j.1365-2583.2010.00997.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The cAMP/PKA signalling pathway and transcription factor cAMP response element-binding protein (CREB) play key roles in long-term memory (LTM) formation. We used two closely related parasitic wasp species, Cotesia glomerata and Cotesia rubecula, which were previously shown to be different in LTM formation, and sequenced at least nine different CREB transcripts in both wasp species. The splicing patterns, functional domains and amino acid sequences were similar to those found in the CREB genes of other organisms. The predicted amino acid sequences of the CREB isoforms were identical in both wasp species. Using real-time quantitative PCR we found that two low abundant CREB transcripts are differentially expressed in the two wasps, whereas the expression levels of high abundant transcripts are similar.

引用

页码：367 / 379

页数：13

共 76 条

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