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Factors influencing hemolytic activity of venom from the jellyfish Rhopilema esculentum Kishinouye

被引：52
作者：
Yu, Huahua
Li, Cuiping
Li, Ronggui
Xing, Ronge
Liu, Song
Li, Pengcheng
机构：
[1] Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
[3] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
来源：
FOOD AND CHEMICAL TOXICOLOGY | 2007年 / 45卷 / 07期
关键词：
hemolytic activity; jellyfish; Rhopilema esculentum; Kishinouye; venom; CHIRONEX-FLECKERI; CARYBDEA-ALATA; PROTEINACEOUS TOXINS; CASSIOPEA-XAMACHANA; NEMATOCYST VENOM; TENTACLE; PURIFICATION; BINDING; STINGS;
D O I：
10.1016/j.fct.2006.12.025
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
In this study, hemolytic activity of venom from the jellyfish Rhopilema esculentum Kishinouye and some factors affecting it were assayed. The HU50 of R. esculentum full venom (RFV) against chicken erythrocytes was 3.40 mu g/ml and a Hill coefficient value was 1.73 suggesting at least two molecules participated in hemolytic activity. The hemolytic activity of RFV was affected by some chemical and physical factors such as divalent cations, EDTA, (NH4)(2)SO4, pH and temperature. In the presence of Mg2+, Cu2+, Zn2+, Fe2+, Ca2+ ( >= 2 mM), Mn2+ (>= 1 mM), EDTA (>= 2 mM) and (NH4)(2)SO4, the hemolytic activity of RFV was reduced. RFV had strong hemolytic activity at the pH 6-10 and the hemolytic ratios were 0.95-1.19. Hemolytic activity was temperature-sensitive and when RFV was pre-incubated at temperatures over 40 degrees C, it was sharply reduced. (c) 2007 Elsevier Ltd. All rights reserved.
引用
收藏
页码：1173 / 1178
页数：6
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