Brefeldin A interferes with peroxisomal protein sorting in the yeast Hansenula polymorpha

被引：49

作者：

Salomons, FA ^{[1
]}

vanderKlei, IJ ^{[1
]}

Kram, AM ^{[1
]}

Harder, W ^{[1
]}

Veenhuis, M ^{[1
]}

机构：

[1] UNIV GRONINGEN,DEPT MICROBIOL,GRONINGEN BIOMOL SCI & BIOENGN INST,CTR BIOL,NL-9751 NN HAREN,NETHERLANDS

来源：

FEBS LETTERS | 1997年 / 411卷 / 01期

关键词：

Brefeldin A; peroxisome; protein sorting; yeast;

D O I：

10.1016/S0014-5793(97)00679-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have studied the effect of brefeldin A (BFA), a fungal toxin that interferes with coated vesicle formation, on the biogenesis of peroxisomes in the yeast Hansenula polymorpha, Addition of BFA (20 mu g/ml) to cultures of H. polymorpha partially inhibited the development of peroxisomes and resulted in the reversible accumulation of newly synthesized peroxisomal membrane and matrix proteins at the endoplasmic reticulum. In contrast, BFA did not interfere with the selective degradation of peroxisomes, Taken together, our data suggest that the ER plays a crucial role in peroxisome biogenesis in H. polymorpha, possibly in the biosynthesis of the peroxisomal membrane, (C) 1997 Federation of European Biochemical Societies.

引用

页码：133 / 139

页数：7

共 39 条

[21] AN OLIGOMERIC PROTEIN IS IMPORTED INTO PEROXISOMES IN-VIVO [J].