Monomeric dark rhodopsin holds the molecular determinants for transducin recognition: Insights from computational analysis

被引：21

作者：

Dell'Orco, Daniele

Seeber, Michele

Fanelli, Francesca

机构：

[1] Dulbecco Telethon Inst, Dept Chem, I-41100 Modena, Italy

[2] Univ Modena, Dept Chem, I-41100 Modena, Italy

来源：

FEBS LETTERS | 2007年 / 581卷 / 05期

关键词：

molecular recognition; docking; GPCRs; G proteins;

D O I：

10.1016/j.febslet.2007.01.074

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this computational study, we have investigated the implications of rhodopsin (Rho) oligomerization in transducin (Gt) recognition. The results of docking simulations between heterotrimeric Gt and monomeric, dimeric and tetrameric inactive Rho corroborate the hypothesis that Rho and Gt can be found coupled already in the dark. Moreover, our extensive computational analysis suggests that the most likely Rho:Gt stoichiometry is the 1:1 one. This means that the essential molecular determinants for Gt recognition and activation are contained in one Rho monomer. In this respect, the complex between one Rho molecule and one heterotrimeric Gt should be considered as the functional unit. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：944 / 948

页数：5

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