Ligation detection reaction-TaqMan procedure for single nucleotide polymorphism detection on genomic DNA

被引：20

作者：

Borodina, TA ^{[1
]}

Lehrach, H ^{[1
]}

Soldatov, AV ^{[1
]}

机构：

[1] Max Planck Inst Mol Genet, D-14195 Berlin, Germany

来源：

ANALYTICAL BIOCHEMISTRY | 2004年 / 333卷 / 02期

关键词：

SNP genotyping; TaqMan; mutation; detection;

D O I：

10.1016/j.ab.2004.05.032

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this article, we describe a genotyping approach applicable to both individual and multiplexed single nucleotide polymorphism (SNP) analysis, based on a ligation detection reaction (LDR) performed directly on genomic DNA. During the ligation, the biallelic state of the SNP locus is converted into a bimarker state of ligated detector oligonucleotides. The state of the markers is then determined by a 5'-nuclease assay (TaqMan) with universal fluorescent probes. The LDR-TaqMan method was successfully applied for the genotyping of 30 SNP loci of Arabidopsis thaliana. The technology is cost-effective, needs no locus-specific optimization, requires minimal manipulations, and has very good potential for automation. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：309 / 319

页数：11

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