U2 small nuclear ribonucleoprotein particle (snRNP) auxiliary factor of 65 kDa, U2AF65, can promote U1 snRNP recruitment to 5′ splice sites

被引：16

作者：

Förch, P ^{[1
]}

Merendino, L ^{[1
]}

Martínez, C ^{[1
]}

Valcárcel, J ^{[1
]}

机构：

[1] European Mol Biol Lab, Gene Express Programme, D-69117 Heidelberg, Germany

来源：

BIOCHEMICAL JOURNAL | 2003年 / 372卷 / 01期

关键词：

arginine- and serine-rich domain (RS domain); psoralen; sex-lethal; splicing; U1 small nuclear ribonucleoparticle (snRNP); U2 auxiliary factor;

D O I：

10.1042/BJ20021202

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The splicing factor U2AF(65) , U2 small nuclear ribonucleoprotein particle (snRNP) auxiliary factor of 65 kDa, binds to pyrimidine-rich sequences at 3' splice sites to recruit U2 snRNP to premRNAs. We report that U2AF 15 can also promote the recruitment of U I snRNP to weak 5' splice sites that are followed by uridine-rich sequences. The arginine- and serine-rich domain of U2AF(65) is critical for U I recruitment, and we discuss the role of its RNA-RNA annealing activity in this novel function of U2AF(65).

引用

页码：235 / 240

页数：6

共 55 条

[1] The splicing factor BBP interacts specifically with the pre-mRNA branchpoint sequence UACUAAC [J].

Berglund, JA ;

Chua, K ;

Abovich, N ;

Reed, R ;

Rosbash, M .

CELL, 1997, 89 (05) :781-787

[2] The RNA-binding protein TIA-1 is a novel mammalian splicing regulator acting through intron sequences adjacent to a 5′ splice site [J].

Del Gato-Konczak, F ;

Bourgeois, CF ;

Le Guiner, C ;

Kister, L ;

Gesnel, MC ;

Stévenin, J ;

Breathnach, R .

MOLECULAR AND CELLULAR BIOLOGY, 2000, 20 (17) :6287-6299

[3] ACCURATE TRANSCRIPTION INITIATION BY RNA POLYMERASE-II IN A SOLUBLE EXTRACT FROM ISOLATED MAMMALIAN NUCLEI [J].

DIGNAM, JD ;

LEBOVITZ, RM ;

ROEDER, RG .

NUCLEIC ACIDS RESEARCH, 1983, 11 (05) :1475-1489

[4] Yeast U1 snRNP-pre-mRNA complex formation without U1snRNA-pre-mRNA base pairing [J].

Du, H ;

Rosbash, M .

RNA, 2001, 7 (01) :133-142

[5] The U1 snRNP protein U1C recognizes the 5′ splice site in the absence of base pairing [J].

Du, HS ;

Rosbash, M .

NATURE, 2002, 419 (6902) :86-90

[6] U2AF(65) recruits a novel human DEAD box protein required for the U2 snRNP-branchpoint interaction [J].

Fleckner, J ;

Zhang, M ;

Valcarcel, J ;

Green, MR .

GENES & DEVELOPMENT, 1997, 11 (14) :1864-1872

[7] The apoptosis-promoting factor TIA-1 is a regulator of alternative pre-mRNA splicing [J].

Förch, P ;

Puig, O ;

Kedersha, N ;

Martínez, C ;

Granneman, S ;

Séraphin, B ;

Anderson, P ;

Valcárcel, J .

MOLECULAR CELL, 2000, 6 (05) :1089-1098

[8] The splicing regulator TIA-1 interacts with U1-C to promote U1 snRNP recruitment to 5′ splice sites [J].

Förch, P ;

Puig, O ;

Martínez, C ;

Séraphin, B ;

Valcárcel, J .

EMBO JOURNAL, 2002, 21 (24) :6882-6892

[9] Modulation of msl-2 5′ splice site recognition by Sex-lethal [J].

Förch, P ;

Merendino, L ;

Martínez, C ;

Valcárcel, J .

RNA, 2001, 7 (09) :1185-1191

[10] Targeting of U2AF(65) to sites of active splicing in the nucleus [J].

GamaCarvalho, M ;

Krauss, RD ;

Chiang, LJ ;

Valcarcel, J ;

Green, MR ;

CarmoFonseca, M .

JOURNAL OF CELL BIOLOGY, 1997, 137 (05) :975-987

← 1 2 3 4 5 6 →