U2 small nuclear ribonucleoprotein particle (snRNP) auxiliary factor of 65 kDa, U2AF65, can promote U1 snRNP recruitment to 5′ splice sites

被引：16

作者：

Förch, P ^{[1
]}

Merendino, L ^{[1
]}

Martínez, C ^{[1
]}

Valcárcel, J ^{[1
]}

机构：

[1] European Mol Biol Lab, Gene Express Programme, D-69117 Heidelberg, Germany

来源：

BIOCHEMICAL JOURNAL | 2003年 / 372卷 / 01期

关键词：

arginine- and serine-rich domain (RS domain); psoralen; sex-lethal; splicing; U1 small nuclear ribonucleoparticle (snRNP); U2 auxiliary factor;

D O I：

10.1042/BJ20021202

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The splicing factor U2AF(65) , U2 small nuclear ribonucleoprotein particle (snRNP) auxiliary factor of 65 kDa, binds to pyrimidine-rich sequences at 3' splice sites to recruit U2 snRNP to premRNAs. We report that U2AF 15 can also promote the recruitment of U I snRNP to weak 5' splice sites that are followed by uridine-rich sequences. The arginine- and serine-rich domain of U2AF(65) is critical for U I recruitment, and we discuss the role of its RNA-RNA annealing activity in this novel function of U2AF(65).

引用

页码：235 / 240

页数：6

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