The JAMM motif of human deubiquitinase Poh1 is essential for cell viability

被引：61

作者：

Gallery, Melissa ^{[1
]}

Blank, Jonathan L. ^{[1
]}

Lin, Yinghui ^{[1
]}

Gutierrez, Juan A. ^{[1
]}

Pulido, Jacqueline C. ^{[1
]}

Rappoli, David ^{[1
]}

Badola, Sunita ^{[1
]}

Rolfe, Mark ^{[1
]}

MacBeth, Kyle J. ^{[1
]}

机构：

[1] Millennium Pharmaceut Inc, Cambridge, MA 02139 USA

来源：

MOLECULAR CANCER THERAPEUTICS | 2007年 / 6卷 / 01期

关键词：

D O I：

10.1158/1535-7163.MCT-06-0542

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Poh1 deubiquitinase activity is required for proteolytic processing of polyubiquitinated substrates by the 26S proteasome, linking deubiquitination to complete substrate degradation. Poh1 RNA interference (RNAi) in HeLa cells resulted in a reduction in cell viability and an increase in polyubiquitinated protein levels, supporting the link between Poh1 and the ubiquitin proteasome pathway. To more specifically test for any requirement of the zinc metalloproteinase motif of Poh1 to support cell viability and proteasome function, we developed a RNAi complementation strategy. Effects on cell viability and proteasome activity were assessed in cells with RNAi of endogenous Poh1 and induced expression of wild-type Poh1 or a mutant form of Poh1, in which two conserved histidines of the proposed catalytic site were replaced with alanines. We show that an intact zinc metalloproteinase motif is essential for cell viability and 26S proteasome function. As a required enzymatic component of the proteasome, Poh1 is an intriguing therapeutic drug target for cancer.

引用

页码：262 / 268

页数：7

共 37 条

[21] MPN plus , a putative catalytic motif found in a subset of MPN domain proteins from eukaryotes and prokaryotes, is critical for Rpn11 function [J].