Use of pulsed field gel electrophoresis to determine the source of microbial contamination of central venous catheters

被引：17

作者：

Livesley M.A. ^{[1
]}

Tebbs S.E. ^{[2
]}

Moss H.A. ^{[2
]}

Faroqui M.H. ^{[3
]}

Lambert P.A. ^{[1
]}

Elliott T.S.J. ^{[2
]}

机构：

[1] Dept. Pharmaceutical and Biol. Sci., Aston University, Birmingham, B4 7ET, Aston Triangle

[2] Department of Clinical Microbiology, Queen Elizabeth Hospital, Edgbaston, Birmingham

[3] Dept. of Anaesth. and Intensive Care, Queen Elizabeth Hospital, Edgbaston, Birmingham

来源：

European Journal of Clinical Microbiology and Infectious Diseases | 1998年 / 17卷 / 2期

关键词：

Central venous catheter; Pulsed-field gel electrophoresis;

D O I：

10.1007/BF01682166

中图分类号：

学科分类号：

摘要：

Microorganisms detected in situ on the distal tip of central venous catheters (CVC) within 90 min of insertion were investigated using pulsed-field gel electrophoresis to analyse genomic fragments obtained with the SmaI restriction enzyme. Thirty patients received a triple lumen CVC, which was inserted directly through the skin using the Seldinger technique. In a further 30 patients a triple lumen CVC was inserted through a Swan sheath, thereby avoiding direct contact of the CVC with the skin. Staphylococci were isolated from the distal tips of the catheters in 6 patients (5 who had the CVC inserted directly through the skin and 1 who had the CVC inserted via a Swan sheath.) Twenty-three staphylococcal isolates were also isolated from the insertion equipment and the skin swabs surrounding the insertion site of these six patients. All the isolates were genotyped. In one of the patients the organisms isolated from the skin were identical to those on the CVC tip. In two further patients similar organisms were isolated from the insertion equipment and the patients' skin. These results, in addition to the reduced colonisation rates observed when catheters were introduced through a Swan sheath, support the hypothesis that microorganisms from the skin are impacted onto the CVC tip and the CVC insertion equipment at catheter insertion.

引用

页码：108 / 112

页数：4

共 17 条

[11]

Lina B., Vandenesch F., Etienne J., Kreiswirth B., Fleurette J., Comparison of coagulase-negative staphylococci by pulsed-field gel electrophoresis, FEMS Microbiology Letters, 92, pp. 133-138, (1992)

[12]

Lina B., Forey F., Togaud J.D., Fleurette J., Chronic bacteraemia due to Staphylococcus epidermidis after bone marrow transplantation, Journal of Medical Microbiology, 42, pp. 156-160, (1995)

[13]

Linares J., Dominguez M.A., Martin R., Current laboratory techniques in the diagnosis of catheter related infections, Nutrition, 13, SUPPL., pp. 10-14, (1997)

[14]

Zaidi A.K.M., Harrell L.J., Rost J.R., Reller L.B., Assessment of similarity among coagulase-negative staphylococci from sequential blood cultures of neonates and children by pulsed-field gel electrophoresis, Journal of Infectious Diseases, 174, pp. 1010-1014, (1996)

[15]

Tenover F.C., Arbeit R.D., Goering R.V., Mickelsen P.A., Murray B.E., Persing D.H., Swaminathan B., Interpreting chromosomal DNA restriction patterns produced by pulsed field gel electrophoresis:criteria for bacterial strain typing, Journal of Clinical Microbiology, 33, pp. 2233-2239, (1995)

[16]

Tebbs S.E., Elliott T.S.J., A novel antimicrobial central venous catheter impregnated with benzalkonium chloride, Journal of Antimicrobial Chemotherapy, 31, pp. 261-271, (1993)

[17]

Liu W.K., Tebbs S.E., Byrne P.O., Elliott T.S.J., The effects of electric current on bacteria colonising intravenous catheters, Journal of Infection, 27, pp. 261-269, (1993)

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