Grabbing the cat by the tail: manipulating molecules one by one

被引：286

作者：

Bustamante C. ^{[1
,2
,3
]}

Macosko J.C. ^{[2
]}

Wuite G.J.L. ^{[3
]}

机构：

[1] Howard Hughes Medical Institute, University of California, Berkeley

[2] Dept. of Molec. and Cell Biolology, University of California, Berkeley

[3] Department of Physics, University of California, Berkeley

来源：

Nature Reviews Molecular Cell Biology | 2000年 / 1卷 / 2期

关键词：

D O I：

10.1038/35040072

中图分类号：

学科分类号：

摘要：

Methods for manipulating single molecules are yielding new information about both the forces that hold biomolecules together and the mechanics of molecular motors. We describe here the physical principles behind these methods, and discuss their capabilities and current limitations.

引用

页码：130 / 136

页数：6

共 66 条

[61]

Muramoto K., Et al., Rotational fluctuation of the sodium-driven flagellar motor of Vibrio alginolyticus induced by binding of inhibitors, J. Mol. Biol., 259, pp. 687-695, (1996)

[62]

Chand A., Viani M.B., Schaffer T.E., Hansma P.K., Microfabricated small metal cantilevers with silicon tip for atomic force microscopy, J. Microelectromech. Sys., 9, pp. 112-116, (2000)

[63]

Osheroff N., Shelton E.R., Brutlag D.L., DNA topoisomerase II from Drosophila melanogaster. Relaxation of supercoiled DNA, J. Biol. Chem., 258, pp. 9536-9543, (1983)

[64]

Fisher T.E., Oberhauser A.F., Carrion-Vazquez M., Marszalek P.E., Fernandez J.M., The study of protein mechanics with the atomic force microscope, Trends Biochem. Sci., 24, pp. 379-384, (1999)

[65]

Florin E.L., Moy V.T., Gaub H.E., Adhesion forces between individual ligand-receptor pairs, Science, 264, pp. 415-417, (1994)

[66]

Lee G.U., Chrisey L.A., Colton R.J., Direct measurement of the forces between complementary strands of DNA, Science, 266, pp. 771-773, (1994)

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