Inter-Simple Sequence Repeat fingerprints to assess genetic diversity in Tunisian fig (Ficus carica L.) germplasm

被引：40

作者：

Amel S.-H. ^{[1
]}

Mokhtar T. ^{[1
]}

Salwa Z. ^{[1
]}

Jihène H. ^{[1
]}

Messaoud M. ^{[1
]}

Abdelmajid R. ^{[1
]}

Mohamed M. ^{[1
]}

机构：

[1] Faculté des Sciences de Tunis, Lab. Genet. Molec., Immunol. B., Campus Universitaire El Manar

来源：

Genetic Resources and Crop Evolution | 2004年 / 51卷 / 3期

关键词：

Ficus carica L; Genetic diversity; Germplasm collection; ISSR;

D O I：

10.1023/B:GRES.0000024012.59828.6b

中图分类号：

学科分类号：

摘要：

The genetic diversity of 18 Tunisian fig cultivars was investigated at the DNA level using the Inter Simple Sequence Repeat (ISSR) associated with the Polymerase Chain Reaction (PCR). Using a set of primers, the most informative ones were selected that were characterized by an important Resolving power value of 29.6. A total of 47 discernible fragments were scored from samples, with a mean of 11.7 fragments per primer. The 90.4% of sample that were polymorphic were scored as molecular markers to examine the Tunisian fig germplasm polymorphism at DNA level. A large genetic diversity as related to ISSR patterns was found within the local Tunisian fig germplasm. An UPGMA dendrogram exhibits the unstructured variability in this crop. Moreover, the principal component analysis shows that the observed diversity was typically continuous. Our data provide a large number of ISSR markers that are useful in the fingerprinting of Ficus carica L. cultivars, and in the understanding of the genetic relationships among these accessions.

引用

页码：269 / 275

页数：6

共 32 条

[11]

Hedfi J., Trifi M., Hannachi Salhi A., Ould Mohamed Salem A., Rhouma A., Marrakchi M., Morphological and isoenzymatic polymorphism in Tunisian fig collection, Second International Symposium on Fig, (2001)

[12]

Joshi S.P., Gupta V.S., Aggarwal R.K., Ranjkar P.K., Brar D.S., Genetic diversity and phylogenetic relationship as revealed by inter simple sequence repeat (ISSR) polymorphism in the genus Oryza, Theor. Appl. Genet., 100, pp. 1311-1320, (2000)

[13]

Khadari B., Lashermes Ph., Kjellberg F., RAPD fingerprints for identification and genetic characterization of fig (Ficus carica L.) genotypes, J. Genet. & Breed., 49, pp. 77-86, (1995)

[14]

Khadari B., Hochu I., Santoni S., Oukabli A., Ater M., Roger J.P., Et al., Which molecular markers are best suited to identify fig cultivars. A comparison of RAPD, ISSR and microsatellite markers, Second International Symposium on Fig, (2001)

[15]

Kjellberg F., Gouyon P.H., Ibrahim M., Raymond M., The stability of the symbiosis between dioecious figs and their pollinators. A study of Ficus carica L. and Blastophaga psenes L., Evolution, 41, pp. 603-704, (1987)

[16]

Lai J.A., Yang W.C., Hsiao J.Y., An assessment of genetic relationships in cultivated teas clones and native wild tea in Taiwan using RAPD and ISSR markers, Bot. Bull. Acad. Sc., 42, pp. 93-100, (2001)

[17]

Mars M., Carraut A., Marrakchi M., Gouiaa M., Gaaliche F., Ressources génétiques fruitières en Tunisie (poirier, oranger, figuier, grenadier), Plant Genet. Res. Newsletter, 100, pp. 14-17, (1994)

[18]

Mars M., Chebli T., Marrakchi M., Multivariate analysis of fig (Ficus carica L.) germplasm in southern Tunisia, Acta Hort., 480, pp. 75-81, (1998)

[19]

Mars M., Fig (Ficus carica L.) genetic resources and breeding, a review, Second International Symposium on Fig, (2001)

[20]

Nei M., Li W.H., Mathematical model for studying genetical variation in terms of restriction endonucleases, Proc. Natl. Acad. Sci., 74, pp. 5267-5273, (1979)

← 1 2 3 4 →