Histone deacetylase inhibitors such as sodium butyrate and trichostatin A induce apoptosis through an increase of the bcl-2-related protein Bad.

被引：82

作者：

Sawa H. ^{[1
]}

Murakami H. ^{[1
]}

Ohshima Y. ^{[1
]}

Sugino T. ^{[1
]}

Nakajyo T. ^{[1
]}

Kisanuki T. ^{[1
]}

Tamura Y. ^{[1
]}

Satone A. ^{[1
]}

Ide W. ^{[1
]}

Hashimoto I. ^{[1
]}

Kamada H. ^{[1
]}

机构：

[1] Oncology Research Center, Hokuto Hospital, Obihiro, ORC282, Hokkaido

来源：

Brain Tumor Pathology | 2001年 / 18卷 / 2期

关键词：

Histone deacetylase inhibitors; Sodium butyrate; Trichostatin A; Glioma; Apoptosis; Bcl-2-related proteins; Bad;

D O I：

10.1007/BF02479423

中图分类号：

学科分类号：

摘要：

The effects of sodium butyrate (SB) and trichostatin A (TSA) on cell proliferation andapoptosis against human glioma T98G, U251MG, and U877MG cells were investigated. Upon exposure to either SB or TSA, cell proliferation was reduced, and apoptosis detected by DNA fragmentation analysis and the cleavage of CPP32 was induced. Previously, we reported that SB increased the expression levels of p21 (WAF-1) and inhibited G1-S transition of the cell cycle. In this study, we showed that TSA also increased p21 expression, suggesting that histone deacetylase (HDAC) inhibitors may up-regulate p21 protein in common and thus arrest proliferation in the G1 phase of the cell cycle. To further determine the underlying molecular mechanisms of apoptosis with either SB or TSA treatment, we studied the expression levels of apoptosis-related proteins in human glioma cells. SB increased the expression of the Bad protein, although the expression of Bcl-2, Bcl-xL, Bax, and Fas was not changed by theaddition of SB. TSA treatment also up-regulated the expression of Bad protein. The results suggest that HDAC inhibitors such as SB and TSA induce apoptosis through an increase in Bad protein in human glioma cells in vitro.

引用

页码：109 / 114

页数：5

共 71 条

[21] Sakata K(1997)The significant role of telomerase activity in human brain tumors Cancer 80 471-476
[22] Armstrong RC(2001)Synergistic growth inhibition of prostate cancer cells by 1 alpha, 25 dihydroxyvitamin D(3) and its 19-nor-hexafluoride analogs in combination with either sodium butyrate or trichostatin A Oncogene 20 1860-1872
[23] Aja T(2001)Effects of retinoic acid and sodium butyrate on gene expression, histone acetylation and inhibition of proliferation of melanoma cells Cancer Lett 163 103-107
[24] Xiang J(1998)Cancer cell sensitization of fas-mediated apoptosis by sodium butyrate Cell Death Differ 5 480-487
[25] El-Deiry WS(undefined)undefined undefined undefined undefined-undefined
[26] Harper JW(undefined)undefined undefined undefined undefined-undefined
[27] O'Connor PM(undefined)undefined undefined undefined undefined-undefined
[28] Michieli P(undefined)undefined undefined undefined undefined-undefined
[29] Chedid M(undefined)undefined undefined undefined undefined-undefined
[30] Lin D(undefined)undefined undefined undefined undefined-undefined

← 1 2 3 4 5 6 7 8 →