Camptothecin and 10-hydroxycamptothecin in callus and plantlets of t Camptotheca acuminata

被引:17
作者
Helmut Wiedenfeld
Miroslawa Furmanowa
Erhard Roeder
Joanna Guzewska
Wlodzimierz Gustowski
机构
[1] Pharmazeutisches Institut der Universität Bonn,Department of Biology and Pharmaceutical Botany
[2] Medical Academy,undefined
来源
Plant Cell, Tissue and Organ Culture | 1997年 / 49卷
关键词
in vitro culture;
D O I
暂无
中图分类号
学科分类号
摘要
The process of callus induction, organogenesis and plantlets regeneration of Camptotheca acuminata Decne is reported. The highest growth rate of callus was observed on MS medium with 1 mg l−1 NAA, 1 mg l−1 kinetin and 60 g l−1 sucrose. All tissues and organs developed in vitro contain camptothecin and 10-hydroxycamptothecin. The presence of 10-hydroxycamptothecin in shoots and callus of Camptotheca acuminata Decne is reported for the first time. The alkaloids were detected and identified using HPLC methods.
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页码:213 / 218
页数:5
相关论文
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