Camptothecin and 10-hydroxycamptothecin in callus and plantlets of t Camptotheca acuminata

被引：17

作者：

Helmut Wiedenfeld

Miroslawa Furmanowa

Erhard Roeder

Joanna Guzewska

Wlodzimierz Gustowski

机构：

[1] Pharmazeutisches Institut der Universität Bonn,Department of Biology and Pharmaceutical Botany

[2] Medical Academy,undefined

来源：

Plant Cell, Tissue and Organ Culture | 1997年 / 49卷

关键词：

in vitro culture;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

The process of callus induction, organogenesis and plantlets regeneration of Camptotheca acuminata Decne is reported. The highest growth rate of callus was observed on MS medium with 1 mg l−1 NAA, 1 mg l−1 kinetin and 60 g l−1 sucrose. All tissues and organs developed in vitro contain camptothecin and 10-hydroxycamptothecin. The presence of 10-hydroxycamptothecin in shoots and callus of Camptotheca acuminata Decne is reported for the first time. The alkaloids were detected and identified using HPLC methods.

引用

页码：213 / 218

页数：5